Endocrine

, Volume 37, Issue 2, pp 274–279

Proteasome activity correlates with male BMI and contributes to the differentiation of adipocyte in hADSC

  • Kozue Sakamoto
  • Youichi Sato
  • Masako Sei
  • Ashraf A. Ewis
  • Yutaka Nakahori
Original Article

DOI: 10.1007/s12020-009-9298-4

Cite this article as:
Sakamoto, K., Sato, Y., Sei, M. et al. Endocr (2010) 37: 274. doi:10.1007/s12020-009-9298-4

Abstract

We have previously reported that 26S proteasome subunit mRNA expressions correlate with male body mass index (BMI). In this study, to investigate whether proteasome activities are correlated with BMI, we recruited 61 healthy young Japanese male subjects, measured proteasome activities in their plasma, and correlated them with their BMI and various metabolic factors. We found that among three different proteasome activities, chymotrypsin-like activity in plasma was positively correlated with BMI in healthy Japanese male subjects. Furthermore, we analyzed proteasome activity in vitro during the differentiation of human adipose-derived stem cell (hADSC) into mature adipocytes. In the early stage of differentiation, proteasome activity was at its highest level, and proteasome inhibitor could inhibit hADSC adipocyte differentiation. Our findings suggest that proteasome is an important controlling factor for the development of obesity and adipogenesis.

Keywords

Proteasome activities Obesity BMI Adipogenesis hADSC 

Supplementary material

12020_2009_9298_MOESM1_ESM.doc (30 kb)
Supplementary material 1 (DOC 31 kb)
12020_2009_9298_MOESM2_ESM.doc (52 kb)
Supplementary material 2 (DOC 52 kb)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kozue Sakamoto
    • 1
  • Youichi Sato
    • 1
  • Masako Sei
    • 1
  • Ashraf A. Ewis
    • 1
  • Yutaka Nakahori
    • 1
  1. 1.Department of Human Genetics and Public Health, Institute of Health Biosciences The University of Tokushima Graduate SchoolTokushimaJapan