Endocrine

, Volume 33, Issue 3, pp 331–337

Berberine inhibits the expression of TNFα, MCP-1, and IL-6 in AcLDL-stimulated macrophages through PPARγ pathway

Authors

  • F. L. Chen
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
    • Department of EndocrinologyNo. 3 People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
  • Z. H. Yang
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
  • Y. Liu
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
  • L. X. Li
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
  • W. C. Liang
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
  • X. C. Wang
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
  • W. B. Zhou
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
  • Y. H. Yang
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
    • Department of Endocrinology, Huashan HospitalFudan University
    • Institute of Endocrinology and DiabetologyFudan University
Original Paper

DOI: 10.1007/s12020-008-9089-3

Cite this article as:
Chen, F.L., Yang, Z.H., Liu, Y. et al. Endocr (2008) 33: 331. doi:10.1007/s12020-008-9089-3

Abstract

Macrophages are the main source of cytokines in atherosclerotic plaques. Modified low-density lipoproteins may stimulate macrophages to produce large quantities of proinflammatory cytokines that promote atherosclerosis. Berberine is the main component of the traditional Chinese medicine umbellatine, which has a widespread effect and was used to treat many diseases clinically. Our previous study found that berberine could increase adipophilin expression in macrophages, which is a target gene of PPARγ. PPARγ agonist could decrease proinflammatory cytokines in macrophage. In this study, we investigated the effects and the mechanism of action of berberine on the expression and secretion of TNFα, MCP-1, and IL-6 in vitro to identify new pharmacological actions of berberine. The results of RT-PCR and ELISA shows that berberine may inhibit the expression and secretion of the tumor necrosis factor α (TNFα), monocyte chemoattractant protein 1 (MCP-1), and interleukin-6 (IL-6) in macrophages stimulated by acetylated low-density lipoprotein (AcLDL), whereas the peroxisome proliferator-activated receptor γ (PPARγ) inhibitor GW9662 could attenuate this effect of berberine. This study demonstrates that berberine may inhibit the expression and production of TNF-α, MCP-1, and IL-6 in AcLDL-stimulated macrophages. This effect might be partially mediated through PPARγ activity.

Keywords

BerberineMacrophageAcetylated low-density lipoproteinInflammationPeroxisome proliferator-activated receptor γ

Copyright information

© Springer Science+Business Media, LLC 2008