Stem Cell Reviews and Reports

, Volume 9, Issue 5, pp 700–708

Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells

  • Jianyu Wang
  • Qi Gu
  • Jie Hao
  • Yundan Jia
  • Binghua Xue
  • Honghong Jin
  • Jing Ma
  • Renyue Wei
  • Tang Hai
  • Qingran Kong
  • Gerelchimeg Bou
  • Ping Xia
  • Qi Zhou
  • Liu Wang
  • Zhonghua Liu
Article

DOI: 10.1007/s12015-013-9439-2

Cite this article as:
Wang, J., Gu, Q., Hao, J. et al. Stem Cell Rev and Rep (2013) 9: 700. doi:10.1007/s12015-013-9439-2

Abstract

Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with Oct4 (Pou5f1), Sox2, Klf4 and c-Myc (termed OSKM, 4 F). Here, we found two other factors (Tbx3 and Nr5α2, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly, Nr5α2 alone could induce piPSCs formation in the presence or absence of c-Myc. These results suggested that Tbx3 and Nr5α2 may have vital roles in Sus scrofa and proposed new insights into pig pluripotent stem cells.

Keywords

piPSCs Nr5α2 Tbx3 Pluripotency Efficiency 

Supplementary material

12015_2013_9439_MOESM1_ESM.pptx (573 kb)
Supplement Figure 1Characterization of 4 F piPSCs. (A) Immunostaining for pluripotency markers of 4 F piPSCs. Positive OCT4 (green) and SOX2 (green) were observed. DNA was stained with Hoechst 33342 (blue). (B) Karyotype of 4 F piPSCs. More than 80 % of the cells showed normal pig karyotype of 38 chromosomes. (C) Embryoid body formation detection. Bars scale, 200 μm. (PPTX 573 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jianyu Wang
    • 1
    • 2
  • Qi Gu
    • 1
    • 2
  • Jie Hao
    • 2
  • Yundan Jia
    • 2
  • Binghua Xue
    • 1
  • Honghong Jin
    • 1
  • Jing Ma
    • 1
  • Renyue Wei
    • 1
  • Tang Hai
    • 2
  • Qingran Kong
    • 1
  • Gerelchimeg Bou
    • 1
  • Ping Xia
    • 1
  • Qi Zhou
    • 2
  • Liu Wang
    • 2
  • Zhonghua Liu
    • 1
  1. 1.College of Life ScienceNortheast Agricultural University of ChinaHarbinPeople’s Republic China
  2. 2.State Key Laboratory of Reproductive Biology, Institute of ZoologyChinese Academy of SciencesBeijingChina

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