Stem Cell Reviews and Reports

, Volume 8, Issue 4, pp 1120–1128

Stage-Specific Cardiomyocyte Differentiation Method for H7 and H9 Human Embryonic Stem Cells

Article

DOI: 10.1007/s12015-012-9403-6

Cite this article as:
Sa, S. & McCloskey, K.E. Stem Cell Rev and Rep (2012) 8: 1120. doi:10.1007/s12015-012-9403-6

Abstract

The generation of cardiomyocytes from human embryonic stem cells (hESC) boasts a variety of potential applications including cell transplantation for myocardial repair. Unfortunately, advancements in the field has been challenged by the low efficiency of cardiomyocyte differentiation from hESC. Recently, Kattman et al. 2011 showed that individual hESC lines require a precise balance of the Activin A and BMP4 signaling for efficient cardiac differentiation. This group also presented differentiation protocols for several human and mouse ESC lines, however; two of the most utilized hESC lines, the H9 and H7 ESC, were not included. Here, we provide protocols, based on the work from Kattman et al. 2011, for generating cardiomyoctyes from H7 and H9 hESC. These hESC line-specific protocols reproducibly direct approximately 50 % of hESC towards the cardiac lineage.

Keywords

Human embryonic stem cellsCardiomyocytesStem cell differentiationEmbryoid bodyActivin ABMP4KDRPDGFR-α

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Graduate Group in Biological Engineering & Small-scale TechnologiesUniversity of California, MercedMercedUSA
  2. 2.School of EngineeringUniversity of California, MercedMercedUSA