Stem Cell Reviews and Reports

, Volume 9, Issue 3, pp 339–349

Comparison of Cardiac Stem Cells and Mesenchymal Stem Cells Transplantation on the Cardiac Electrophysiology in Rats with Myocardial Infarction

Authors

  • Shao-Xin Zheng
    • Cardiovascular MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology
  • Yin-Lun Weng
    • Emergency MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University
  • Chang-Qing Zhou
    • Emergency MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University
  • Zhu-Zhi Wen
    • Cardiovascular MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology
  • Hui Huang
    • Cardiovascular MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology
  • Wei Wu
    • Cardiovascular MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology
    • Cardiovascular MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology
    • Cardiovascular & Emergency MedicineThe Sun Yat-sen Memorial Hospital of Sun Yat-sen University. Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology
    • Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of EducationSun Yat-Sen University
Article

DOI: 10.1007/s12015-012-9367-6

Cite this article as:
Zheng, S., Weng, Y., Zhou, C. et al. Stem Cell Rev and Rep (2013) 9: 339. doi:10.1007/s12015-012-9367-6

Abstract

Introduction

Whether transplanted cardiac stem cells (CSCs) and mesenchymal stem cells (MSCs) improved ventricular fibrillation threshold (VFT) similarly is still unclear. We sought to compare the effects of the CSC and MSC transplantation on the electrophysiological characteristics and VFT in rats with myocardial infarction (MI).

Methods

MI was induced in 30 male Sprague–Dawley rats. Two weeks later, animals were randomized to receive 5 × 106 CSCs labeled with PKH26 in PBS or 5 × 106 MSCs labeled with PKH26 in phosphate buffer solution(PBS) or PBS alone injection into the infarcted anterior ventricular free wall. Six weeks after the injection, electrophysiological characteristics and VFT were measured. Labeled CSCs and MSCs were observed in 5 μm cryostat sections from each heart.

Results

Malignant ventricular arrhythmias were significantly (P = 0.0055) less inducible in the CSC group than the MSC group. The VFTs were improved in the CSC group compared with the MSC group. Labeled CSCs and MSCs were identified in the infarct zone and infarct marginal zone. Labeled CSCs expressed Connexin-43, von Willebrand factor, α-smooth muscle actin and α-sarcomeric actin,while the Labeled MSCs expressed von Willebrand factor, α-smooth muscle actin and α-sarcomeric actin in vivo.

Conclusions

After 6 weeks of cell transplantation, CSCs are superior to MSCs in modulating the electrophysiological abnormality and improving the VFT in rats with MI. CSCs and MSCs express markers that suggest muscle, endothelium and vascular smooth muscle phenotypes in vivo, but MSCs rarely express Connexin-43.

Keywords

Cardiac stem cellsMesenchymal stem cellsElectrophysiological characteristicsMyocardial infarctionVentricular fibrillation threshold

Abbreviations

ART

Activation recovery time

ARTd

ART dispersion

ARTc

Correct ART

ARTcd

ARTc dispersion

Cx43

Connexin-43

CSCs

Cardiac stem cells

MSCs

Mesenchymal stem cells

MI

Myocardial infarction

PBS

Phosphate buffer solution

VT

Ventricular tachycardia

VFT

Ventricular fibrillation threshold

VF

Ventricular fibrillation

Copyright information

© Springer Science+Business Media, LLC 2012