Stem Cell Reviews and Reports

, Volume 7, Issue 3, pp 616–623

Delayed Recovery of Myocardial Blood Flow After Intracoronary Stem Cell Administration

  • Mariann Gyöngyösi
  • Rayyan Hemetsberger
  • Susanne Wolbank
  • Valentin Pichler
  • Christoph Kaun
  • Aniko Posa
  • Zsolt Petrasi
  • Örs Petnehazy
  • Renate Hofer-Warbinek
  • Rainer de Martin
  • Florian Gruber
  • Imre Benedek
  • Teodora Benedek
  • Istvan Kovacs
  • Istvan BenedekJr.
  • Christian A. Plass
  • Silvia Charwat
  • Gerald Maurer
Article

DOI: 10.1007/s12015-010-9213-7

Cite this article as:
Gyöngyösi, M., Hemetsberger, R., Wolbank, S. et al. Stem Cell Rev and Rep (2011) 7: 616. doi:10.1007/s12015-010-9213-7
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Abstract

The aim of the present study was to investigate the changes in absolute myocardial blood flow (AMF) after intracoronary injections of mesenchymal SC (MSC) and compared to controls in closed-chest reperfused acute myocardial infarction (AMI) in pigs. Male MSCs, transiently transfected with Luciferase (Luc-MSC) were delivered \( \left( {{9}.{7}\pm {1}.{2} \times {1}{0^{{6}}}} \right) \) intracoronary in the open infarct-related artery one-week post-AMI in female pigs (group MSC), while saline was injected with the same injection rate in controls (group C). The AMF was measured immediately after, and 3, 12 and 24 h post-intracoronary Luc-MSC or saline injections. In vitro bioluminescence images and quantitative real-time TaqMan PCR measurements were performed to quantify the sex-mismatched MSCs. No difference between the groups was observed regarding the weight, heart rate, blood pressure and global ejection fraction 1-week post-AMI. The baseline AMF were similar in the groups (61.3 ± 15. vs 61.1 ± 12.0 ml/min). AMF was decreased significantly immediately after intracoronary MSC delivery (42.0 ± 12.4 vs 57.7 ± 15.7 ml/min p = 0.013), and remained low at 3 h (40.9 ± 13.4 vs 55.8 ± 4.9 ml/min, p = 0.004), 12 h (43.0 ± 3.7 vs 57.8 ± 5.4 ml/min, p = 0.001) with incomplete recovery at 24 h (47.2 ± 5.5 vs 62.1 ± 14.1 ml/min, p = 0.038) as compared to controls, respectively. In vitro bioluminescence displayed transfected Luc-MSCs along the proximal and mid part of the LAD, with limited number (295 ± 101 sry copied/million cardiac cells) of Y-chromosome-MSCs in the infarcted area. Intracoronary injection of SCs results in immediate decrease of AMF, with delayed recovery. The delivery of the SC into the injured myocardium might be hindered by the altered coronary pressure and flow conditions.

Keywords

Myocardial infarction Blood flow Catheterization Imaging Stem cells 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Mariann Gyöngyösi
    • 1
  • Rayyan Hemetsberger
    • 1
  • Susanne Wolbank
    • 2
  • Valentin Pichler
    • 2
  • Christoph Kaun
    • 1
  • Aniko Posa
    • 1
  • Zsolt Petrasi
    • 3
  • Örs Petnehazy
    • 3
  • Renate Hofer-Warbinek
    • 4
  • Rainer de Martin
    • 4
  • Florian Gruber
    • 5
  • Imre Benedek
    • 6
  • Teodora Benedek
    • 6
  • Istvan Kovacs
    • 6
  • Istvan BenedekJr.
    • 6
  • Christian A. Plass
    • 1
  • Silvia Charwat
    • 1
  • Gerald Maurer
    • 1
  1. 1.Department of CardiologyMedical University of ViennaViennaAustria
  2. 2.Ludwig Boltzmann Institute for Clinical and Experimental Traumatology /AUVA Research Center Austrian Cluster for Tissue RegenerationViennaAustria
  3. 3.Institute of Diagnostic Imaging and Radiation OncologyUniversity of KaposvarKaposvarHungary
  4. 4.Department of Biomolecular Medicine and Pharmacology, Institute of Vascular Biology and Thrombosis ResearchMedical University of ViennaViennaAustria
  5. 5.Department of DermatologyMedical University of ViennaViennaAustria
  6. 6.Department of CardiologyUniversity of Targu MuresTargu MuresRomania

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