Article

Stem Cell Reviews and Reports

, Volume 7, Issue 2, pp 269-291

The Potential of Adipose Stem Cells in Regenerative Medicine

  • Bettina LindroosAffiliated withRegea—Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital Email author 
  • , Riitta SuuronenAffiliated withRegea—Institute for Regenerative Medicine, University of Tampere and Tampere University HospitalDepartment of Eye, Ear and Oral Diseases, Tampere University HospitalDepartment of Biomedical Engineering, Tampere University of Technology
  • , Susanna MiettinenAffiliated withRegea—Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital

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Abstract

Adipose stem cells (ASCs) are an attractive and abundant stem cell source with therapeutic applicability in diverse fields for the repair and regeneration of acute and chronically damaged tissues. Importantly, unlike the human bone marrow stromal/stem stem cells (BMSCs) that are present at low frequency in the bone marrow, ASCs can be retrieved in high number from either liposuction aspirates or subcutaneous adipose tissue fragments and can easily be expanded in vitro. ASCs display properties similar to that observed in BMSCs and, upon induction, undergo at least osteogenic, chondrogenic, adipogenic and neurogenic, differentiation in vitro. Furthermore, ASCs have been shown to be immunoprivileged, prevent severe graft-versus-host disease in vitro and in vivo and to be genetically stable in long-term culture. They have also proven applicability in other functions, such as providing hematopoietic support and gene transfer. Due to these characteristics, ASCs have rapidly advanced into clinical trials for treatment of a broad range of conditions. As cell therapies are becoming more frequent, clinical laboratories following good manufacturing practices are needed. At the same time as laboratory processes become more extensive, the need for control in the processing laboratory grows consequently involving a greater risk of complications and possibly adverse events for the recipient. Therefore, the safety, reproducibility and quality of the stem cells must thoroughly be examined prior to extensive use in clinical applications. In this review, some of the aspects of examination on ASCs in vitro and the utilization of ASCs in clinical studies are discussed.

Keywords

Adipose stem cells Stem cell therapy Good manufacturing practice Defined serum-free culturing conditions Flow cytometry