Stem Cell Reviews and Reports

, Volume 6, Issue 2, pp 248–259

Suspension Culture of Undifferentiated Human Embryonic and Induced Pluripotent Stem Cells

  • Michal Amit
  • Judith Chebath
  • Victoria Margulets
  • Ilana Laevsky
  • Yael Miropolsky
  • Kohava Shariki
  • Meital Peri
  • Idit Blais
  • Guy Slutsky
  • Michel Revel
  • Joseph Itskovitz-Eldor
Article

DOI: 10.1007/s12015-010-9149-y

Cite this article as:
Amit, M., Chebath, J., Margulets, V. et al. Stem Cell Rev and Rep (2010) 6: 248. doi:10.1007/s12015-010-9149-y

Abstract

Alongside their contribution to research, human embryonic stem cells (hESC) may also prove valuable for cell-based therapies. Traditionally, these cells have been grown in adhesion culture either with or without feeder cells, allowing for their continuous growth as undifferentiated cells. However, to be applicable in therapy and industry they must be produced in a scalable and controlled process. Here we present for the first time a suspension culture system for undifferentiated hESC and induced pluripotent stem cells (iPSC), based on medium supplemented with the IL6RIL6 chimera (interleukin-6 receptor fused to interleukin-6), and basic fibroblast growth factor. Four hESC lines cultured in this system maintained all ESC features after 20 passages, including stable karyotype and pluripotency. Similar results were obtained when hESC were replaced with iPSC from two different cell lines. We demonstrate that the IL6RIL6 chimera supports the self-renewal and expansion of undifferentiated hESC and iPSC in suspension, and thus present another efficient system for large-scale propagation of undifferentiated pluripotent cells for clinical and translational applications.

Keywords

Embryonic stem cellsInduced pluripotent stem cellsIL6-IL6 receptor chimeragp1303DSuspensionBioreactor

Supplementary material

12015_2010_9149_MOESM1_ESM.docx (5 mb)
ESM 1(DOCX 4.96MB)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Michal Amit
    • 1
  • Judith Chebath
    • 2
  • Victoria Margulets
    • 1
  • Ilana Laevsky
    • 1
  • Yael Miropolsky
    • 1
  • Kohava Shariki
    • 1
  • Meital Peri
    • 1
  • Idit Blais
    • 1
  • Guy Slutsky
    • 2
  • Michel Revel
    • 2
  • Joseph Itskovitz-Eldor
    • 1
  1. 1.Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, and the Sohnis and Forman Families Stem Cell Center, the Bruce Rappaport Faculty of MedicineTechnion-Israel Institute of TechnologyHaifaIsrael
  2. 2.Department of Molecular GeneticsWeizmann Institute of ScienceRehovotIsrael