, Volume 6, Issue 2, pp 270-281
Date: 08 Apr 2010

Robust Enhancement of Neural Differentiation from Human ES and iPS Cells Regardless of their Innate Difference in Differentiation Propensity

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Abstract

Our analyses of three human induced pluripotent stem cell (hiPSC) and six human embryonic stem cell (hESC) lines showed marked variability in differentiation potential into specific lineages, which often hampers their differentiation into specific cell types or cell lineages of interest. Simultaneous inhibition of both Activin/Nodal and BMP pathways with small molecules, SB431542 and dorsomorphin (DM), respectively, promoted significant neural differentiation from all human pluripotent stem cell (hPSC) lines tested, regardless of their differentiation propensity. On the contrary, differentiation into other cell lineages and the number of undifferentiated cells were significantly reduced after differentiation by the dual inhibition. These results demonstrate that innate differentiation propensity of hPSCs could be overcome, at least in part, by modulation of intracellular signaling pathways, resulting in efficient generation of desirable cell types, such as neural cells.

Authors contributions:

D-.S.K.: Conception and design, Collection and/or assembly of data, Data analysis and interpretation, Manuscript writing
J. S. L.: Collection and/or assembly of data, Data analysis and interpretation
J.W.L.: Data analysis and interpretation
J.Y.K.: Collection and/or assembly of data, Data analysis and interpretation
Y.J.H.: Collection and/or assembly of data, Data analysis and interpretation
H-.S.K.: Data analysis and interpretation, Manuscript writing
D-.Y.H.: Data analysis and interpretation, Manuscript writing
I-.H.P.: Provision of study materials, Data analysis and interpretation
G.Q.D.: Provision of study materials, Data analysis and interpretation
D-.W.K.: Conception and design, Financial support, Data analysis and interpretation, Manuscript writing and Final approval of manuscript
An erratum to this article can be found at http://dx.doi.org/10.1007/s12015-010-9151-4