Cell Biochemistry and Biophysics

, Volume 68, Issue 2, pp 283–290

MiR-335, an Adipogenesis-Related MicroRNA, is Involved in Adipose Tissue Inflammation

  • Lu Zhu
  • Ling Chen
  • Chun-Mei Shi
  • Guang-Feng Xu
  • Lu-Lian Xu
  • Ling-Ling Zhu
  • Xi-Rong Guo
  • Yuhui Ni
  • Yan Cui
  • Chenbo Ji
Original Paper

DOI: 10.1007/s12013-013-9708-3

Cite this article as:
Zhu, L., Chen, L., Shi, CM. et al. Cell Biochem Biophys (2014) 68: 283. doi:10.1007/s12013-013-9708-3

Abstract

During the development of obesity, adipose tissue releases a host of different adipokines and inflammatory cytokines, such as leptin, resistin, tumor necrosis factor α (TNF-α), Interleukin-6 (IL-6), and adiponectin, which mediate insulin resistance. Recently, some microRNAs (miRNAs) regulated by adiponectin were identified as novel targets for controlling adipose tissue inflammation. Therefore, the relationship between adipokines and miRNA is worth studying. MiR-335 is an adipogenesis-related miRNA and implicated in both fatty acid metabolism and lipogenesis. In this study, we focused on the association of miR-335 and adipokines, and examined the expression trend of miR-335 during human adipocyte differentiation. Our results showed that miR-335 is significantly upregulated with treatment of leptin, resistin, TNF-α, and IL-6 in human mature adipocytes, and its expression elevated in the process of adipocyte differentiation. Interestingly, the transcriptional regulation of miR-335 by these adipokines seems independent of its host gene (mesoderm-specific transcript homolog, MEST). Thus, we cloned and identified potential promoter of miR-335 within the intron of MEST. As a result, a fragment about 600-bp length upstream sequences of miR-335 had apparent transcription activity. These findings indicated a novel role for miR-335 in adipose tissue inflammation, and miR-335 might play an important role in the process of obesity complications via its own transcription mechanism.

Keywords

Adipocyte MicroRNA Adipokines Inflammatory cytokines Obesity Insulin resistance 

Supplementary material

12013_2013_9708_MOESM1_ESM.tif (395 kb)
Supplementary material 1 (TIFF 395 kb). The construct of pTB-Cherry vector. This vector was designed for analyzing the promoter activity by observing red fluorescence under fluorescence microscope. We blocked TA minimal promoter (PTA) and replaced luciferase (Luc) gene using mCherry gene to form the 3861bp pTB-Cherry vector
12013_2013_9708_MOESM2_ESM.doc (30 kb)
Supplementary material 2 (DOC 29 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Lu Zhu
    • 1
  • Ling Chen
    • 2
    • 3
  • Chun-Mei Shi
    • 2
    • 3
  • Guang-Feng Xu
    • 2
  • Lu-Lian Xu
    • 2
  • Ling-Ling Zhu
    • 2
  • Xi-Rong Guo
    • 2
    • 3
  • Yuhui Ni
    • 3
  • Yan Cui
    • 1
  • Chenbo Ji
    • 2
    • 3
  1. 1.School of NursingNanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.Institute of Pediatrics, Nanjing Medical UniversityNanjingPeople’s Republic of China
  3. 3.Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical UniversityNanjingPeople’s Republic of China