Cell Biochemistry and Biophysics

, Volume 68, Issue 2, pp 247–257

Resveratrol Induces Pro-oxidant Effects and Time-Dependent Resistance to Cytotoxicity in Activated Hepatic Stellate Cells

  • Leo A. Meira Martins
  • Bárbara P. Coelho
  • Guilherme Behr
  • Letícia F. Pettenuzzo
  • Izabel C. C. Souza
  • José Cláudio F. Moreira
  • Radovan Borojevic
  • Carmem Gottfried
  • Fátima Costa Rodrigues Guma
Original Paper

DOI: 10.1007/s12013-013-9703-8

Cite this article as:
Martins, L.A.M., Coelho, B.P., Behr, G. et al. Cell Biochem Biophys (2014) 68: 247. doi:10.1007/s12013-013-9703-8

Abstract

Resveratrol (RSV) is known for its antioxidant properties; however, this compound has been proposed to have cytotoxic and pro-oxidant effects depending on its concentration and time of exposure. We previously reported the cell cycle arrest effect of low doses of RSV in GRX cells, an activated hepatic stellate cell model. Here, we evaluated the effects of RSV treatment (0.1–50 μM) for 24 and 120 h on GRX viability and oxidative status. Only treatment with 50 μM of RSV reduced the amount of live cells. However, even low doses of RSV induced an increased reactive species production at both treatment times. While being diminished within 24 h, RSV induced an increase in the SOD activity in 120 h. The cellular damage was substantially increased at 24 h in the 50 μM RSV-treated group, as indicated by the high lipoperoxidation, which may be related to the significant cell death and low proliferation. Paradoxically, this cellular damage and lipoperoxidation were considerably reduced in this group after 120 h of treatment while the surviving cells proliferated. In conclusion, RSV induced a dose-dependent pro-oxidant effect in GRX cells. The highest RSV dose induced oxidative-related damage, drastically reducing cell viability; but this cytotoxicity seems to be attenuated during 120 h of treatment.

Keywords

Cell viability Catalase Hepatic stellate cells Lipoperoxidation Liver fibrosis Resveratrol Superoxide dismutase Oxidative stress 

Abbreviations

CAT

Catalase

ECM

Extracellular matrix

HSC

Hepatic stellate cells

ROS

Reactive oxygen species

RSV

Resveratrol

SOD

Superoxide dismutase

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Leo A. Meira Martins
    • 1
  • Bárbara P. Coelho
    • 1
  • Guilherme Behr
    • 1
  • Letícia F. Pettenuzzo
    • 1
  • Izabel C. C. Souza
    • 1
  • José Cláudio F. Moreira
    • 1
  • Radovan Borojevic
    • 2
  • Carmem Gottfried
    • 1
  • Fátima Costa Rodrigues Guma
    • 1
  1. 1.Department of BiochemistryICBS, Federal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Department of Histology and EmbriologyICB, PABCAM, Federal University of Rio de Janeiro (UFRJ)Rio de JaneiroBrazil