Cell Biochemistry and Biophysics

, Volume 67, Issue 2, pp 255–262

Epigenetic Signature of Early Cardiac Regulatory Genes in Native Human Adipose-Derived Stem Cells

  • Alice Pasini
  • Francesca Bonafè
  • Marco Govoni
  • Carlo Guarnieri
  • Paolo G. Morselli
  • Hari S. Sharma
  • Claudio M. Caldarera
  • Claudio Muscari
  • Emanuele Giordano
Original Paper

DOI: 10.1007/s12013-013-9610-z

Cite this article as:
Pasini, A., Bonafè, F., Govoni, M. et al. Cell Biochem Biophys (2013) 67: 255. doi:10.1007/s12013-013-9610-z

Abstract

Adipose-derived stem cells (ADSCs) are stromal mesenchymal stem cells isolated from lipoaspirates, and they display a broad potential to differentiate toward different lineages. The role of epigenetics in regulating the expression of their lineage-specific genes is under evaluation, however till date virtually nothing is known about the relative significance of cardiac-specific transcription factor genes in human ADSCs. The aim of this study was to investigate DNA promoter methylation and relevant histone modifications involving MEF-2C, GATA-4, and Nkx2.5 in native human ADSCs. CpG sites at the transcription start in their promoters were found unmethylated using methylation-specific PCR. Chromatin immunoprecipitation assay showed low levels of total acetylated H3 histone (acH3) and high levels of trimethylated lysine 27 in H3 histone (H3K27me3) which were associated with both GATA-4 and Nkx2.5 promoters, indicating their transcriptional repressive chromatin arrangement. On the other hand, the opposite was apparent for MEF-2C promoter. Accordingly, MEF-2C—but not GATA-4 and Nkx2.5—transcripts were evidenced in native human ADSCs. These results suggest that the chromatin arrangement of these early cardiac regulatory genes could be explored as a level of intervention to address the differentiation of human ADSCs toward the cardiac lineage.

Keywords

DNA methylationHistone modificationsChromatinEpigeneticsAdipose-derived stem cellsEarly cardiac regulatory genes

Abbreviations

ADSCs

Adipose-derived stem cells

TF

Transcription factor

MSP

Methylation-specific PCR

ChIP

Chromatin immunoprecipitation

RT-PCR

Reverse transcription PCR

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Alice Pasini
    • 1
  • Francesca Bonafè
    • 2
  • Marco Govoni
    • 3
  • Carlo Guarnieri
    • 2
    • 3
  • Paolo G. Morselli
    • 4
  • Hari S. Sharma
    • 5
  • Claudio M. Caldarera
    • 6
  • Claudio Muscari
    • 2
    • 3
  • Emanuele Giordano
    • 1
    • 2
    • 3
  1. 1.Laboratory of Cellular and Molecular Engineering “S. Cavalcanti”University of BolognaCesenaItaly
  2. 2.Department of Biochemistry “G. Moruzzi”University of BolognaBolognaItaly
  3. 3.Health Science and Technology, Interdepartmental Center for Industrial ResearchUniversity of BolognaBolognaItaly
  4. 4.School of Plastic SurgeryUniversity of BolognaBolognaItaly
  5. 5.Department of PathologyVU University Medical CenterAmsterdamThe Netherlands
  6. 6.National Institute for Cardiovascular ResearchBolognaItaly