Cell Biochemistry and Biophysics

, Volume 61, Issue 2, pp 277–287

Investigation of siRNA-Loaded Polyethylenimine-Coated Human Serum Albumin Nanoparticle Complexes for the Treatment of Breast Cancer

Original Paper

DOI: 10.1007/s12013-011-9201-9

Cite this article as:
Abbasi, S., Paul, A. & Prakash, S. Cell Biochem Biophys (2011) 61: 277. doi:10.1007/s12013-011-9201-9
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Abstract

Small interfering RNA (siRNA) molecules have great potential for developing into a future therapy for breast cancer. To overcome the issues related to rapid degradation and low transfection of naked siRNA, polyethylenimine (PEI)-coated human serum albumin (HSA) nanoparticles have been characterized and studied here for efficient siRNA delivery to the MCF-7 breast cancer cell line. The optimized nanoparticles were ~90 nm in size, carrying a surface charge of +26 mV and a polydispersity index (PDI) less than 0.25. The shape and morphology of the particles was studied using electron microscopy. A cytotoxicity assessment of the nanoparticles showed no correlation of cytotoxicity with HSA concentration, while using high molecular weight PEI (MW of 70 against 25 kDa) showed higher cytotoxicity. The optimal transfection achieved of fluorescin-tagged siRNA loaded into PEI-coated HSA nanoparticles was 61.66 ± 6.8%, prepared with 6.25 μg of PEI (25 kDa) added per mg of HSA and 20 mg/ml HSA, indicating that this nonviral vector may serve as a promising gene delivery system.

Keywords

Nanoparticles Nanomedicine Breast cancer Gene silencing Albumin Nanobiotechnology 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Artificial Cells and Organs Research CentreFaculty of Medicine, McGill UniversityMontrealCanada

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