Cardiovascular Toxicology

, Volume 11, Issue 1, pp 67–73

Homocysteine Induces Oxidative–Nitrative Stress in Heart of Rats: Prevention by Folic Acid

  • Janaína Kolling
  • Emilene B. Scherer
  • Aline Andrea da Cunha
  • Maira Jaqueline da Cunha
  • Angela T. S. Wyse
Article

DOI: 10.1007/s12012-010-9094-7

Cite this article as:
Kolling, J., Scherer, E.B., da Cunha, A.A. et al. Cardiovasc Toxicol (2011) 11: 67. doi:10.1007/s12012-010-9094-7

Abstract

Hyperhomocysteinemia is a risk factor for cardiovascular disease, stroke, and thrombosis; however, the mechanisms by which homocysteine triggers these dysfunctions are not fully understood. In the present study, we investigated the effect of chronic hyperhomocysteinemia on some parameters of oxidative stress, namely thiobarbituric acid reactive substances, an index of lipid peroxidation, 2′,7′-dichlorofluorescein (H2DCF) oxidation, activities of antioxidant enzymes named superoxide dismutase and catalase, as well as nitrite levels in heart of young rats. We also evaluated the effect of folic acid on biochemical alterations elicited by hyperhomocysteinemia. Wistar rats received daily subcutaneous injection of homocysteine (0.3–0.6 μmol/g body weight) and/or folic acid (0.011 μmol/g body weight) from their 6th to the 28th day of life. Controls and treated rats were killed 1 h and/or 12 h after the last injection. Results showed that chronic homocysteine administration increases lipid peroxidation and reactive species production and decreases enzymatic antioxidant defenses and nitrite levels in the heart of young rats killed 1 h, but not 12 h after the last injection of homocysteine. Folic acid concurrent administration prevented homocysteine effects probable by its antioxidant properties. Our data indicate that oxidative stress is elicited by chronic hyperhomocystenemia, a mechanism that may contribute, at least in part, to the cardiovascular alterations characteristic of hyperhomocysteinemic patients. If confirmed in human beings, our results could propose that the supplementation of folic acid can be used as an adjuvant therapy in cardiovascular alterations caused by homocysteine.

Keywords

HyperhomocysteinemiaHomocysteineOxidative stressVascular damageCardiovascular disease

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Janaína Kolling
    • 1
    • 2
  • Emilene B. Scherer
    • 1
    • 2
  • Aline Andrea da Cunha
    • 1
    • 2
  • Maira Jaqueline da Cunha
    • 1
    • 2
  • Angela T. S. Wyse
    • 1
    • 2
  1. 1.Laboratório de Neuroproteção e Doenças MetabólicasICBS, Universidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Laboratório de Erros Inatos do Metabolismo, Departamento de BioquímicaICBS, Universidade Federal do Rio Grande do SulPorto AlegreBrazil