Biological Trace Element Research

, Volume 146, Issue 2, pp 281–286

Iron, Copper, and Zinc Transport: Inhibition of Divalent Metal Transporter 1 (DMT1) and Human Copper Transporter 1 (hCTR1) by shRNA

  • Alejandra Espinoza
  • Solange Le Blanc
  • Manuel Olivares
  • Fernando Pizarro
  • Manuel Ruz
  • Miguel Arredondo
Article

DOI: 10.1007/s12011-011-9243-2

Cite this article as:
Espinoza, A., Le Blanc, S., Olivares, M. et al. Biol Trace Elem Res (2012) 146: 281. doi:10.1007/s12011-011-9243-2

Abstract

Iron (Fe), copper (Cu), and zinc (Zn) fulfill various essential biological functions and are vital for all living organisms. They play important roles in oxygen transport, cell growth and differentiation, neurotransmitter synthesis, myelination, and synaptic transmission. Because of their role in many critical functions, they are commonly used in food fortification and supplementation strategies globally. To determine the involvement of divalent metal transporter 1 (DMT1) and human copper transporter 1 (hCTR1) on Fe, Cu, and Zn uptake, Caco-2 cells were transfected with four different shRNA plasmids to selectively inhibit DMT1 or hCTR1 transporter expression. Fe and Cu uptake and total Zn content measurements were performed in shRNA-DMT1 and shRNA-hCTR1 cells. Both shRNA-DMT1 and shRNA-hCTR1 cells had lower apical Fe uptake (a decrease of 51% and 41%, respectively), Cu uptake (a decrease of 25.8% and 38.5%, respectively), and Zn content (a decrease of 23.1% and 22.7%, respectively) compared to control cells. These results confirm that DMT1 is involved in active transport of Fe, Cu, and Zn although Zn showed a different relative capacity. These results also show that hCTR1 is able to transport Fe and Zn.

Keywords

Iron Copper Zinc DMT1 hCTR1 shRNA 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Alejandra Espinoza
    • 1
  • Solange Le Blanc
    • 1
  • Manuel Olivares
    • 1
  • Fernando Pizarro
    • 1
  • Manuel Ruz
    • 2
  • Miguel Arredondo
    • 1
  1. 1.Micronutrient Laboratory, Institute of Nutrition and Food TechnologyUniversity of ChileMaculChile
  2. 2.Department of Nutrition, Faculty of MedicineUniversity of ChileSantiagoChile