Article

Biological Trace Element Research

, Volume 144, Issue 1, pp 1405-1418

First online:

Investigations on the Interaction between Cuprous Oxide Nanocubes and Bovine Serum Albumin with Comprehensive Spectroscopic Methods

  • Peng JuAffiliated withCollege of Chemistry and Material Science, Shandong Agricultural University
  • , Hai FanAffiliated withCollege of Chemistry and Material Science, Shandong Agricultural University
  • , Tao LiuAffiliated withCollege of Chemistry and Material Science, Shandong Agricultural University
  • , Lin CuiAffiliated withCollege of Chemistry and Material Science, Shandong Agricultural University
  • , Shiyun AiAffiliated withCollege of Chemistry and Material Science, Shandong Agricultural University Email author 
  • , Xiaona WuAffiliated withCollege of Chemistry and Material Science, Shandong Agricultural University

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Abstract

The interaction between cuprous oxide (Cu2O) nanocubes and bovine serum albumin (BSA) was investigated from a spectroscopic angle under simulative physiological conditions. Under pH 7.4, Cu2O could effectively quench the intrinsic fluorescence of BSA via static quenching. The apparent binding constant (K A) was 3.23, 1.91, and 1.20 × 104 M-1 at 298, 304, and 310 K, respectively, and the number of binding sites was 1.05. According to the Van’t Hoff equation, the thermodynamic parameters (ΔH° = −63.39 kJ mol-1, ΔS° = −126.45 J mol-1 K-1) indicated that hydrogen bonds and van der Waals forces played a major role in stabilizing the BSA–Cu2O complex. Besides, the average binding distance (r 0 = 2.76 nm) and the critical energy transfer distance (R 0 = 2.35 nm) between Cu2O and BSA were also evaluated according to Förster’s non-radioactive energy transfer theory. Furthermore, UV-visible and circular dichroism results showed that the addition of Cu2O changed the secondary structure of BSA and led to a decrease in α-helix. All results showed that BSA underwent substantial conformational changes induced by Cu2O, which can be very helpful in the study of nanomaterials in the application of biomaterials.

Keywords

Cu2O nanocubes BSA Fluorescence quenching Interaction