Biological Trace Element Research

, Volume 143, Issue 1, pp 394–402

Effects of Zinc Supplementation and Deficiency on Bone Metabolism and Related Gene Expression in Rat

  • Jian-Yi Sun
  • Jian-Feng Wang
  • Nai-Tao Zi
  • Ming-Yan Jing
  • Xiao-Yan Weng

DOI: 10.1007/s12011-010-8869-9

Cite this article as:
Sun, JY., Wang, JF., Zi, NT. et al. Biol Trace Elem Res (2011) 143: 394. doi:10.1007/s12011-010-8869-9


One hundred male rats were randomly divided into four groups (n = 25) and fed a Zn-adequate diet (ZA, 46.39 mg/kg), Zn-deficient diet (ZD, 3.20 mg/kg), Zn-overdose diet (ZO, 234.39 mg/kg), or were pair-fed a Zn-adequate diet (PF) for 5 weeks, respectively. The body weight, femur weight, and activity of alkaline phosphatase (ALP) were reduced in the ZD group but were increased in the ZO group. Zn concentrations in both liver and femur were elevated in the ZO group, whereas femur Zn was decreased in the ZD group. The concentrations of calcium and phosphorus were lower in the ZD than those in other groups. Serum calcium concentration was decreased in the ZD. The relative expression level of ALP was decreased in both ZD and PF, and no significant differences were observed between ZO and ZA. Insulin-like growth factor-I (IGF-I) mRNA level was reduced in the ZD but unchanged in the ZO and PF group. Zn deficiency also decreased ALP mRNA level as compared with that of PF group. Carbonic anhydrase II mRNA level was not affected by Zn. Nevertheless, dietary Zn influenced the growth, bone metabolism, and expression of IGF-I and ALP in male growing rats.


Zinc Femur Insulin-like growth factor-I Carbonic anhydrase Bone metabolism Gene expression 



Alkaline phosphatase


Body weight


Carbonic anhydrase II


Insulin-like growth factor-I





Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jian-Yi Sun
    • 1
  • Jian-Feng Wang
    • 2
  • Nai-Tao Zi
    • 1
  • Ming-Yan Jing
    • 1
    • 4
  • Xiao-Yan Weng
    • 3
  1. 1.The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal ScienceZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Zhejiang Radio and TV UniversityHangzhouPeople’s Republic of China
  3. 3.College of Life ScienceZhejiang UniversityHangzhouPeople’s Republic of China
  4. 4.Department of Animal ScienceUniversity of ManitobaWinnipegCanada

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