Biological Trace Element Research

, Volume 141, Issue 1, pp 131–149

Evaluation of Status of Trace and Toxic Metals in Biological Samples (Scalp Hair, Blood, and Urine) of Normal and Anemic Children of Two Age Groups

  • Faheem Shah
  • Tasneem Gul Kazi
  • Hassan Imran Afridi
  • Naveed Kazi
  • Jameel Ahmed Baig
  • Abdul Qadir Shah
  • Sumaira Khan
  • Nida Fatima Kolachi
  • Sham Kumar Wadhwa
Article

DOI: 10.1007/s12011-010-8736-8

Cite this article as:
Shah, F., Kazi, T.G., Afridi, H.I. et al. Biol Trace Elem Res (2011) 141: 131. doi:10.1007/s12011-010-8736-8

Abstract

Anemia affects a substantial portion of the world’s population, provoking severe health problems as well as important economic losses to the region in which this condition is found. This study was designed to compare the levels of essential trace and toxic elements in scalp hair, blood, and urine samples of anemic children (n = 132) with age range 1–5 and 6–10 years of both genders. For a comparative study, 134 non-anemic age- and sex-matched children as control subjects, residing in the same city, were selected. The metals in the biological samples were measured by flame atomic absorption spectrophotometry/electrothermal atomic absorption spectrometry prior to microwave-assisted acid digestion. The proposed method was validated using certified reference samples of hair, blood, and urine. The results indicated significantly lower levels of iron, copper, and zinc in the biological samples as compared to the control children of both genders (p = 0.01–0.008). The mean values of lead and cadmium were significantly high in all three biological samples of anemic children as compared to non-anemic children of both age groups (p = 0.005–0.001). The ratios of essential metal to toxic metals in the biological samples of anemic children of both age groups were significantly lower than that of controls. Deficiency of essential trace metals and high level of toxic metals may play a role in the development of anemia in the subjects under study.

Keywords

AnemiaEssential and toxic metalsBiological samplesChildren

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Faheem Shah
    • 1
  • Tasneem Gul Kazi
    • 1
  • Hassan Imran Afridi
    • 1
  • Naveed Kazi
    • 2
  • Jameel Ahmed Baig
    • 1
  • Abdul Qadir Shah
    • 1
  • Sumaira Khan
    • 1
  • Nida Fatima Kolachi
    • 1
  • Sham Kumar Wadhwa
    • 1
  1. 1.National Center of Excellence in Analytical ChemistryUniversity of SindhJamshoroPakistan
  2. 2.Liaquat University of Medical and Health SciencesJamshoroPakistan