Biological Trace Element Research

, Volume 126, Issue 1, pp 222–236

Gastroprotective and Anti-oxidative Properties of Ascorbic Acid on Indomethacin-induced Gastric Injuries in Rats


DOI: 10.1007/s12011-008-8205-9

Cite this article as:
Koc, M., Imik, H. & Odabasoglu, F. Biol Trace Elem Res (2008) 126: 222. doi:10.1007/s12011-008-8205-9


Reactive oxygen species (ROS) have been implicated in the etiology of indomethacin-induced gastric mucosal damage. This study investigated ascorbic acid (vitamin C)’s protective effects against oxidative gastric mucosal damage induced by indomethacin. Ascorbic acid is a powerful antioxidant because it can donate a hydrogen atom and form a relatively stable ascorbyl free radical. We have investigated alterations in the levels of myeloperoxidase, antioxidant system enzymes (glutathione S-transferase, superoxide dismutase, glutathione reductase, catalase, glutathione peroxidase), lipid peroxidation and glutathione, as markers for ulceration process following oral administration of ascorbic acid, famotidine, lansoprazole, and ranitidine in rats with indomethacin-induced ulcers. In the present study, we found that (1) ascorbic acid, famotidine, lansoprazole and ranitidine reduced the development of indomethacin-induced gastric damages; (2) the administration of indomethacin caused a significant decrease in the levels of superoxide dismutase, glutathione peroxidase, glutathione S-transferase and glutathione, and an increase in the lipid peroxidation level; (3) the administration of ascorbic acid reversed the trend, inducing a significant increase of these enzymes’ levels and a reduction in lipid peroxidation level in tissues; and (4) catalase, glutathione reductase and myeloperoxidase activities, increased by indomethacin, were found to be lower in the ascorbic acid, famotidine, lansoprazole and ranitidine-treated groups. The results indicate that the gastroprotective properties of ascorbic acid could be related to its positive effects on the antioxidant system and myeloperoxidase activity in indomethacin-induced gastric ulcers in rats.


Ascorbic acid Gastroprotective effect Antioxidant enzymes Myeloperoxidase Neutrophil infiltration Indomethacin 

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  1. 1.Faculty of Art and Sciences, Department of BiologyBozok UniversityYozgatTurkey
  2. 2.Faculty of Veterinary Sciences, Department of Animal Husbandry and Animal NutritionAtaturk UniversityErzurumTurkey
  3. 3.Faculty of Pharmacy, Department of BiochemistryAtaturk UniversityErzurumTurkey