Biological Trace Element Research

, Volume 118, Issue 3, pp 233–243

Transport of Intranasally Instilled Fine Fe2O3 Particles into the Brain: Micro-distribution, Chemical States, and Histopathological Observation

Authors

  • Bing Wang
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
  • Meng Wang
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
  • Jun W. Shi
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
  • Fang Zhang
    • Graduate School of Chinese Academy of Sciences
  • Hong Ouyang
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
  • Yu L. Zhao
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
  • Zhi F. Chai
    • Laboratory for Bio-Environmental Effects of Nanomaterials and Nanosafety and Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy PhysicsChinese Academy of Sciences
    • Institute of Nuclear TechnologyShenzhen University
  • Yu Y. Huang
    • Beijing Synchrotron Radiation Laboratory, Institute of High Energy PhysicsChinese Academy of Sciences
  • Ya N. Xie
    • Beijing Synchrotron Radiation Laboratory, Institute of High Energy PhysicsChinese Academy of Sciences
  • Hai F. Wang
    • College of Chemistry and Molecular EngineeringPeking University
  • Jing Wang
    • College of Chemistry and Molecular EngineeringPeking University
Article

DOI: 10.1007/s12011-007-0028-6

Cite this article as:
Wang, B., Feng, W.Y., Wang, M. et al. Biol Trace Elem Res (2007) 118: 233. doi:10.1007/s12011-007-0028-6

Abstract

It has been demonstrated that inhaled fine (d < 2.5 μm) and ultrafine (d < 100 nm) particles produce more severe toxicity than coarse particles. Some recent data support the concept that the central nervous system (CNS) may be a target for the inhaled fine particulates. This work describes initial observation of the transport of intranasally instilled fine ferric oxide (Fe2O3) particles in animal brain. The iron micro-distribution and chemical state in the mice olfactory bulb and brain stem on day 14 after intranasal instillation of fine Fe2O3 particle (280 ± 80 nm) suspension at a single dose of 40 mg/kg body weight were analyzed by synchrotron radiation x-ray fluorescence and x-ray absorption near-edge structure (XANES). The micro-distribution map of iron in the olfactory bulb and brain stem shows an obvious increase of Fe contents in the olfactory nerve and the trigeminus of brain stem, suggesting that Fe2O3 particles were possibly transported via uptake by sensory nerve endings of the olfactory nerve and trigeminus. The XANES results indicate that the ratios of Fe (III)/Fe (II) were increased in the olfactory bulb and brain stem. The further histopathological observation showed that the neuron fatty degeneration occurred in the CA3 area of hippocampus. Such results imply an adverse impact of inhalation of fine Fe2O3 particles on CNS.

Keywords

Fine ferric oxide particle Intranasal instillation Olfactory transportation Brain Micro-distribution Chemical state Pathological observation

Copyright information

© Humana Press Inc. 2007