Abstract
In the present study, hepatoprotective and antioxidant properties of aqueous extract of Phyllanthus fraternus (AEPF 200, 300, and 400 mg/kg body weight (bw), orally) were investigated against cyclophosphamide (CPA 200 mg/kg, bw, intraperitoneally administered) induced liver damage in mice. Histopathological studies of CPA administration cause liver injury, featuring substantial increase in serum glutamic oxaloacetic transaminase, serum glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, acid phosphatase, and total bilirubin. Moreover, CPA intoxication also causes strong oxidative stress, which is evident from significant increase in lipid peroxide level. These changes were coupled with a decline in superoxide dismutase and catalase as well as albumin, cholesterol level, red blood cell (RBC), and white blood cell (WBC) count. AEPF-treated mice displayed a significant inhibition of lipid peroxidation and augmentation of endogenous antioxidants. The study emphasizes the hepatoprotective effect of AEPF against CPA-induced oxidative liver injury, which may serve as a promising medicinal herb in complementary chemotherapeutic modalities.
Similar content being viewed by others
References
Dollery, C. (1999). Cyclophosphamide. In C. Dollery (Ed.), Therapeutic drugs (pp. 349–353). Edinburgh: Churchill Livingstone.
Hui, M. K. C., Wu, W. K. K., Shin, V. Y., So, W. H. L., & Cho, C. H. (2006). International Journal Medical Science, 3, 1–6.
DeLeve, L. D. (1996). Hepatology, 24, 830–837.
Ansari, R. A., Tripathi, S. C., Patnaik, G. K., & Dhawan, B. N. (1991). Journal of Ethnopharmacology, 34, 61–68.
Sriover, D. C., Weisdorf, S., Bloomer, J., McGlave, P., & Weisdorf, D. (1989). Hepatology, 9, 443.
Das, U. B., Mallick, M., Debnath, J. M., & Ghosh, D. (2002). Asian Journal of Andrology, 4, 201–207.
Stankiewicz, A., Skrzydlewska, E., & Makiela, M. (2002). Drug Metabolism and Drug Interaction, 19, 67–82.
Verma, N., & Vinayak, M. (2008). Molecular Biology Reports, 35, 535–540.
Ahmad, N., Abbasi, B. H., Rahman, I. U., & Fazal, H. (2013). Applied Biochemistry and Biotechnology, 169, 2004–2015.
Gill, S. S., & Tuteja, N. (2010). Plant Physiology and Biochemistry, 48, 909–930.
Panda, V., Ashar, H. D., & Sharan, A. (2013). Botanics: Target and Therapy, 3, 27–37.
Calixto, J. B., Santos, A. R., Cechinel, F. V., & Yunes, R. A. (1998). Medicinal Research Reviews, 4, 225–258.
Kushwah, A. S., Patil, B. M., & Thippeswamy, B. S. (2010). International Journal of Pharmacology, 6, 624–630.
Matur, B. M., Matthew, T., & Ifeanyi, C. I. C. (2009). New York Science Journal, 2, 12–19.
Colle, D., Priscilla, L., Arantes, G. P., Luz, S. C. A., Athayde, M. L., Rocha, J. B. T. & Soares, F. A. A. (2012). Journal of Medicinal Food, 549-556.
Evans, W. C. (2009). Saunders Elsevier, Edinburgh.
Lindley, C. M., Hamilton, G., McCune, J. S., Faucette, S., Shord, S. S., Hawke, R. L., et al. (2002). Drug Metabolism and Disposition, 30, 814–821.
Pass, G. J., Carrie, D., Boylan, M., Lorimore, S., Wright, E., Houston, B., et al. (2005). Cancer Reserch, 65, 4211–4217.
Paolo, A. D., Danesi, R., & Tacca, M. D. (2004). Pharmacology Research, 49, 331–342.
Colvin, O. M. (1999). Current Pharmacology Design, 5, 555–560.
Scherz-Shouval, R., & Elazar, Z. (2007). Trends Cell Biology, 17, 422–427.
Zhang, Z. F., Fan, S. H., Lu, J., Wu, D. M., Shan, Q., & Hu, B. (2009). Journal of Agricultural and Food Chemistry, 57, 7731–7736.
Hou, Z., Qin, P., & Ren, G. (2010). Journal of Agricultural and Food Chemistry, 58, 3191–3196.
Drotman, R., & Lawhan, G. (1978). Drug Chemistry and Toxicology, 1, 163–171.
Berrigan, M. J., Struck, R. F., & Gurtoo, H. L. (1987). Cancer Biochemistry Biophysics, 9, 265–270.
Pigeolet, E., Corbisier, P., Houbion, A., Lambert, D., Michiels, C., Raes, M., et al. (1990). Mechanism of Ageing Development, 51, 283–297.
Sreelatha, S., Dinesh, E., & Uma, C. (2012). Asian Pacific Journal of Cancer Prevention, 13, 2775–2780.
Acknowledgments
The financial assistance provided by University Grant Commission, New Delhi, to Sangita Singh as SRF and CSIR, New Delhi, to Richa Upadhyay as SRF are gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lata, S., Singh, S., NathTiwari, K. et al. Evaluation of the Antioxidant and Hepatoprotective Effect of Phyllanthus fraternus Against a Chemotherapeutic Drug Cyclophosphamide. Appl Biochem Biotechnol 173, 2163–2173 (2014). https://doi.org/10.1007/s12010-014-1018-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-014-1018-8