Applied Biochemistry and Biotechnology

, Volume 173, Issue 5, pp 1023–1037

Antimicrobial Agents Act Differently on Staphyloccocus aureus and Ralstonia eutropha Flavohemoglobins

  • Aymen Ezzine
  • Myriam Moussaoui
  • Emna El Hammi
  • Mohamed Nejib Marzouki
  • Laura Baciou

DOI: 10.1007/s12010-014-0938-7

Cite this article as:
Ezzine, A., Moussaoui, M., El Hammi, E. et al. Appl Biochem Biotechnol (2014) 173: 1023. doi:10.1007/s12010-014-0938-7


Flavohemoglobins (FlavoHb) play a key role in bacterial resistance to nitrosative stress and NO signaling modulation. In this study, we cloned, expressed, and characterized the flavoHb from the opportunistic pathogen, Staphylococcus aureus. The higher amino-acid sequence homology is shared with that from Saccharomyces cerevisiae which was therefore used to build a model structure by homology modeling. Interestingly, the high sequence homology with S. cerevisiae did not correlate with the enzymatic and kinetic properties which are much similar to those of Escherichia coli. In vitro and aerobically, we showed that S. aureus and Ralstonia eutropha flavoHbs accept cytochrome c and oxygen as substrates. Based on this feature, we investigated the preferences for both substrates depending on miconazole or econazole addition and found that the inhibitor chemical composition is determinant.


Flavohemoglobins Molecular modeling Recombinant expression Staphylococcus aureus Reactive oxygen species 





Flavohemoglobin from Staphylococcus aureus


Flavohemoglobin from Ralstonia eutropha


Flavohemoglobin from Escherichia coli


Flavohemoglobin from Saccharomyces cerevisiae


Superoxide dismutase


Cytochrome c

Supplementary material

12010_2014_938_MOESM1_ESM.pdf (35 kb)
ESM 1(PDF 35 kb)
12010_2014_938_MOESM2_ESM.pdf (989 kb)
ESM 2(PDF 988 kb)
12010_2014_938_MOESM3_ESM.pdf (277 kb)
ESM 3(PDF 276 kb)
12010_2014_938_MOESM4_ESM.pdf (35 kb)
ESM 4(PDF 35 kb)

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Aymen Ezzine
    • 1
  • Myriam Moussaoui
    • 1
  • Emna El Hammi
    • 1
  • Mohamed Nejib Marzouki
    • 2
  • Laura Baciou
    • 1
  1. 1.Laboratoire de Chimie PhysiqueUniversité Paris Sud, UMR8000 CNRS, Faculté des SciencesOrsayFrance
  2. 2.Laboratoire d’ingénierie des protéines et molécules bioactives, Institut National des Sciences Appliquées et de TechnologieUniversité de CarthageTunisTunisia

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