Applied Biochemistry and Biotechnology

, Volume 172, Issue 6, pp 3026–3041

Identification of Potential Vaccine Candidates Against Streptococcus pneumoniae by Reverse Vaccinology Approach

  • Sandipan Talukdar
  • Shubhranshu Zutshi
  • K. S. Prashanth
  • Kandarpa K. Saikia
  • Parveen Kumar
Article

DOI: 10.1007/s12010-014-0749-x

Cite this article as:
Talukdar, S., Zutshi, S., Prashanth, K.S. et al. Appl Biochem Biotechnol (2014) 172: 3026. doi:10.1007/s12010-014-0749-x

Abstract

In the past few decades, genome-based approaches have contributed significantly to vaccine development. Our aim was to identify the most conserved and immunogenic antigens of Streptococcus pneumoniae, which can be potential vaccine candidates in the future. BLASTn was done to identify the most conserved antigens. PSORTb 3.0.2 was run to predict the subcellular localization of the proteins. B cell epitope prediction was done for the immunogenicity testing. Finally, BLASTp was done for verifying the extent of similarity to human proteome to exclude the possibility of autoimmunity. Proteins failing to comply with the set parameters were filtered at each step. Based on the above criteria, out of the initial 22 pneumococcal proteins selected for screening, pavB and pullulanase were the most promising candidate proteins.

Keywords

S. pneumoniaeAntigensImmunogenicpavBPullulanaseVaccine

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sandipan Talukdar
    • 1
  • Shubhranshu Zutshi
    • 2
  • K. S. Prashanth
    • 2
  • Kandarpa K. Saikia
    • 1
  • Parveen Kumar
    • 3
  1. 1.Department of Biotechnology & Bioengineering, ISTGauhati UniversityGuwahatiIndia
  2. 2.Department of Biotechnology, National Institute of Pharmaceutical Education & ResearchGauhati Medical CollegeGuwahatiIndia
  3. 3.Laboratory of Molecular Pharmacology & Toxicology, Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education & ResearchGauhati Medical CollegeGuwahatiIndia