Applied Biochemistry and Biotechnology

, Volume 171, Issue 3, pp 590–615

Predictions of Enzymatic Parameters: A Mini-Review with Focus on Enzymes for Biofuel

Article

DOI: 10.1007/s12010-013-0328-6

Cite this article as:
Yan, S. & Wu, G. Appl Biochem Biotechnol (2013) 171: 590. doi:10.1007/s12010-013-0328-6

Abstract

Enzymatic reactions are very basic processes in biological systems, and parameters related to enzymatic reactions always provide good indicators for understanding of mechanisms underlined in enzymatic reactions, for better controlling of enzymatic reactions, and for comparison of different enzymes. In this mini-review: first, parameters in enzymatic reactions were briefly reviewed from three different standpoints; second, predictions of parameters in enzymatic reactions without information on enzyme structure were shortly reviewed from viewpoints of geometric approach, graphic approach and compartmental approach; third, predictions of parameters in enzymatic reaction with information on enzyme structure were reviewed from the points of view of modeling, with 19 currently available databases, and 17 software packages and web servers; fourth, the current state of prediction on parameters in enzymatic reaction in biofuel industry with respect to cellulolytic enzymes were reviewed; fifth, the pros and cons for future development were discussed; and finally, a worked example was given in the Appendix to describe the whole procedures of prediction of enzymatic parameters in reactions.

Keywords

BiofuelEnzymePredictionReview

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Non-food BiorefineryGuangxi Key Laboratory of Biorefinery, Guangxi Academy of SciencesNanningChina