Applied Biochemistry and Biotechnology

, Volume 169, Issue 6, pp 1774–1789

RNA Interference—A Silent but an Efficient Therapeutic Tool

Authors

  • Puthucode Venkatakrishnan Ramachandran
    • Entomology Research Institute, Loyola College
    • Entomology Research Institute, Loyola College
Article

DOI: 10.1007/s12010-013-0098-1

Cite this article as:
Ramachandran, P.V. & Ignacimuthu, S. Appl Biochem Biotechnol (2013) 169: 1774. doi:10.1007/s12010-013-0098-1

Abstract

RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in comparison with other antisense-based techniques. RNAi can be can be achieved by introducing chemically synthesized small interfering RNAs (siRNAs) into a cell system. A more stable knockdown effect can be brought about by the use of plasmid or viral vectors encoding the siRNA. RNAi has been used in reverse genetics to understand the function of specific genes and also as a therapeutic tool in treating human diseases. This review provides a brief insight into the therapeutic applications of RNAi against debilitating diseases.

Keywords

RNAisiRNAshRNAmiRNACancerOcular diseasesCardiovascular diseasesDiabetes mellitusViral diseasesGene silencingTherapeutic tool

Copyright information

© Springer Science+Business Media New York 2013