Applied Biochemistry and Biotechnology

, Volume 169, Issue 5, pp 1531–1545

Ethanol Production Using Whole Plant Biomass of Jerusalem Artichoke by Kluyveromyces marxianus CBS1555

Article

DOI: 10.1007/s12010-013-0094-5

Cite this article as:
Kim, S., Park, J.M. & Kim, C.H. Appl Biochem Biotechnol (2013) 169: 1531. doi:10.1007/s12010-013-0094-5

Abstract

Jerusalem artichoke is a low-requirement sugar crop containing cellulose and hemicellulose in the stalk and a high content of inulin in the tuber. However, the lignocellulosic component in Jerusalem artichoke stalk reduces the fermentability of the whole plant for efficient bioethanol production. In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, with a 0.924 g/L/h ethanol productivity. These results show that combining the tuber and the stalk hydrolysate is a useful strategy for whole biomass utilization in effective bioethanol fermentation from Jerusalem artichoke.

Keywords

Bioethanol Jerusalem artichoke Stalk Kluyveromyces marxianus Simultaneous saccharification and fermentation 

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Jeonbuk Branch InstituteKorea Research Institute of Bioscience and BiotechnologyJeongeupSouth Korea