Applied Biochemistry and Biotechnology

, Volume 167, Issue 4, pp 776–790

Biosynthesis of Silver Nanoparticles Using Latex from Few Euphorbian Plants and Their Antimicrobial Potential

Article

DOI: 10.1007/s12010-012-9710-z

Cite this article as:
Patil, S.V., Borase, H.P., Patil, C.D. et al. Appl Biochem Biotechnol (2012) 167: 776. doi:10.1007/s12010-012-9710-z

Abstract

The synthesis of well-dispersed and ultrafine metal nanoparticles has great interest due to their distinctive physicochemical properties and biomedical applications. This study is the first report of one-step solvent-free synthesis of AgNPs using Euphorbiaceae plant latex. Among evaluated eight latex-producing plants, four (Jatropha curcas, Jatropha gossypifolia, Pedilanthus tithymaloides, and Euphorbia milii) showed high potential to produce physicochemically distinct, small-sized and bactericidal AgNPs. Phytochemical screening showed presence of rich amount of biochemicals in these plants. J. gossypifolia showed uniformly dispersed comparatively small-sized AgNPs. Dose-dependent growth inhibition of bacterial pathogens Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermis, and Micrococcus luteus was observed for J. gossypifolia latex-synthesized AgNPs with minimum inhibitory concentration values 30, 40, 70, 60, and 60 ppm, respectively, after 24 h. Possible mode of action of AgNPs against pathogens was confirmed by analyzing enzymes and cell leakage.

Keywords

EuphorbiaceaeLatexNanoparticlesAntimicrobialJatropha gossypifoliaZeta potential

Supplementary material

12010_2012_9710_Fig6_ESM.jpg (38 kb)
ESM. 1

(JPEG 37 kb)

12010_2012_9710_MOESM1_ESM.pdf (65 kb)
ESM. 2(PDF 64 kb)
12010_2012_9710_MOESM2_ESM.pdf (65 kb)
ESM. 3(PDF 65 kb)

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.North Maharashtra Microbial Culture Collection Centre (NMCC)North Maharashtra UniversityJalgaonIndia