Applied Biochemistry and Biotechnology

, Volume 166, Issue 6, pp 1423–1432

Effect of Physicochemical Characteristics of Cellulosic Substrates on Enzymatic Hydrolysis by Means of a Multi-Stage Process for Cellobiose Production

  • Caroline Vanderghem
  • Nicolas Jacquet
  • Sabine Danthine
  • Christophe Blecker
  • Michel Paquot
Article

DOI: 10.1007/s12010-011-9535-1

Cite this article as:
Vanderghem, C., Jacquet, N., Danthine, S. et al. Appl Biochem Biotechnol (2012) 166: 1423. doi:10.1007/s12010-011-9535-1

Abstract

The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.

Keywords

CellobioseMultistage processCellulase bindingWater retention valueMicrocrystalline cellulosePaper pulp

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Caroline Vanderghem
    • 1
  • Nicolas Jacquet
    • 1
  • Sabine Danthine
    • 1
  • Christophe Blecker
    • 1
  • Michel Paquot
    • 1
  1. 1.Department of Industrial Biological ChemistryGembloux Agro-Bio Tech, University of LiègeGemblouxBelgium