Applied Biochemistry and Biotechnology

, Volume 164, Issue 5, pp 604–611

Cloning of Two Genes (LAT1,2) Encoding Specific l-Arabinose Transporters of the l-Arabinose Fermenting Yeast Ambrosiozyma monospora

Article

DOI: 10.1007/s12010-011-9161-y

Cite this article as:
Verho, R., Penttilä, M. & Richard, P. Appl Biochem Biotechnol (2011) 164: 604. doi:10.1007/s12010-011-9161-y

Abstract

We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.

Keywords

Sugar transport l-Arabinose transport l-Arabinose metabolism Pentose fermentation Metabolic engineering Yeast 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.VTT Technical Research Centre of FinlandEspooFinland