Vectors for Glucose-Dependent Protein Expression in Saccharomyces cerevisiae
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- Thierfelder, S., Ostermann, K., Göbel, A. et al. Appl Biochem Biotechnol (2011) 163: 954. doi:10.1007/s12010-010-9099-5
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Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119–122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5′-region from either of the following genes: HXK1, YGR243, HXT4 and HXT7. Expression mediated by the respective 5′-regions was monitored with EGFP, yEGFP3-CLN2pest and TurboGFP as marker genes. Fluorescence is induced 2.7-fold using the HXK1, 2.3-fold using the YGR243-, 5-fold using the HXT7- and 12.6-fold using the HXT4 5′-regions upon depletion of glucose to a concentration of <0.5 g/l.