Applied Biochemistry and Biotechnology

, Volume 160, Issue 8, pp 2256–2264

Effect of Additives on the Activity of Tannase from Aspergillus awamori MTCC9299

  • Vinod Chhokar
  • Meenakshi Sangwan
  • Vikas Beniwal
  • Kiran Nehra
  • Kaur S. Nehra
Article

DOI: 10.1007/s12010-009-8813-7

Cite this article as:
Chhokar, V., Sangwan, M., Beniwal, V. et al. Appl Biochem Biotechnol (2010) 160: 2256. doi:10.1007/s12010-009-8813-7

Abstract

Tannase from Aspergillus awamori MTCC 9299 was purified using ammonium sulfate precipitation followed by ion-exchange chromatography. A purification fold of 19.5 with 13.5% yield was obtained. Temperature of 30 °C and pH of 5.5 were found optimum for tannase activity. The effects of metals and organic solvents on the activity of tannase were also studied. Metal ions Mg+2, Mn+2, Ca+2, Na+, and K+ stimulated the tannase activity, while Cu+2, Fe+3, and Co+2 acted as inhibitors of the enzyme. The addition of organic solvents like acetic acid, isoamylalcohol, chloroform, isopropyl alcohol, and ethanol completely inhibited the enzyme activity. However, butanol and benzene increased the enzyme activity.

Keywords

TannaseDEAE-celluloseOrganic solventsMetal ionsAspergillus awamori MTCC 9299

Copyright information

© Humana Press 2009

Authors and Affiliations

  • Vinod Chhokar
    • 1
  • Meenakshi Sangwan
    • 2
  • Vikas Beniwal
    • 1
  • Kiran Nehra
    • 2
  • Kaur S. Nehra
    • 3
  1. 1.Department of Bio and Nano TechnologyGuru Jambheshwar University of Science and TechnologyHisarIndia
  2. 2.Department of BiotechnologyChoudhary Devilal UniversitySirsaIndia
  3. 3.Department of BiotechnologyF.G.M. Govt. CollegeAdampurIndia