Applied Biochemistry and Biotechnology

, Volume 160, Issue 8, pp 2342–2354

Effect of Chain Length of Alcohol on the Lipase-Catalyzed Esterification of Propionic Acid in Supercritical Carbon Dioxide


DOI: 10.1007/s12010-009-8696-7

Cite this article as:
Varma, M.N. & Madras, G. Appl Biochem Biotechnol (2010) 160: 2342. doi:10.1007/s12010-009-8696-7


The esterification of propionic acid was investigated using three different alcohols, namely, isopropyl alcohol, isobutyl alcohol, and isoamyl alcohol. The variation of conversion with time for the synthesis of isoamyl propionate was investigated in the presence of five enzymes. Novozym 435 showed the highest activity, and this was used as the enzyme for investigating the various parameters that influence the esterification reaction. The Ping-Pong Bi-Bi model with inhibition by both acid and alcohol was used to model the experimental data and determine the kinetics of the esterification reaction.


Supercritical carbon dioxide Novozym 435 Esterification Primary alcohol Secondary alcohol Ping-Pong Bi-Bi mechanism 

Copyright information

© Humana Press 2009

Authors and Affiliations

  1. 1.Department of Chemical EngineeringIndian Institute of ScienceBangaloreIndia

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