Article

Applied Biochemistry and Biotechnology

, Volume 160, Issue 5, pp 1341-1355

Alpha-Glucosidase Folding During Urea Denaturation: Enzyme Kinetics and Computational Prediction

  • Xue-Qiang WuAffiliated withDepartment of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University
  • , Jun WangAffiliated withSchool of Medicine, Shenzhen University
  • , Zhi-Rong LüAffiliated withDepartment of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University
  • , Hong-Min TangAffiliated withYangtze Delta Region Institute of Tsinghua University
  • , Daeui ParkAffiliated withKorean BioInformation Center (KOBIC), KRIBB
  • , Sang-Ho OhAffiliated withKorean BioInformation Center (KOBIC), KRIBB
  • , Jong BhakAffiliated withKorean BioInformation Center (KOBIC), KRIBB
  • , Long ShiAffiliated withDepartment of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University
  • , Yong-Doo ParkAffiliated withDepartment of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical UniversityYangtze Delta Region Institute of Tsinghua University
    • , Fei ZouAffiliated withDepartment of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University Email author 

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Abstract

In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increase in the hydrophobic surface of this enzyme induced by urea resulted in aggregation caused by unstable folding intermediates. We also simulated the docking between alpha-glucosidase and urea. The docking simulation suggested that several residues, namely THR9, TRP14, LYS15, THR287, ALA289, ASP338, SER339, and TRP340, interact with urea. Our study provides insights into the alpha-glucosidase unfolding pathway and 3D structure of alpha-glucosidase.

Keywords

Alpha-glucosidase Urea unfolding Docking simulation