Applied Biochemistry and Biotechnology

, Volume 160, Issue 5, pp 1356–1361

Antibacterial Activities of Crude Extract of Aloe barbadensis to Clinically Isolated Bacterial Pathogens


DOI: 10.1007/s12010-009-8577-0

Cite this article as:
Pandey, R. & Mishra, A. Appl Biochem Biotechnol (2010) 160: 1356. doi:10.1007/s12010-009-8577-0


The antibacterial activity of Aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. Enterococcus bovis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Morganella morganii, and Klebsiella pneumoniae causing infection in human being. Ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. Relatively higher MIC concentrations were obtained for gram negative bacteria E. coli and K. pneumoniae, with ethanol extract; however, no inhibitory effect was noted for aqueous extract. Ethanolic extract possesses great inhibitory activity for gram positive bacteria, E. bovis followed by S. aureus. Among gram negative bacteria, highest inhibitory effect was observed with P. aeruginosa, followed by M. morganii, P. mirabilis, and P. vulgaris, which was significant (p < 0.01) than E. coli and K. pneumoniae. Antimicrobial activity tests of crude extract of A. barbadensis were carried out to validate the use of traditional medicinal herbal and results of this study tend to give credence to the common use of A. barbadensis gel and leaf.


Aloe vera Antibacterial Ethanolic extract Herbal Medicinal plant 

Supplementary material

12010_2009_8577_MOESM1_ESM.pdf (712 kb)
ESM 1(PDF 712 KB)

Copyright information

© Humana Press 2009

Authors and Affiliations

  1. 1.Department of Biotechnology, Institute of Allied Health (Paramedical) Services, Education and TrainingUttarakhand Forest Hospital TrustHaldwaniIndia
  2. 2.G. B. Pant University of Agriculture and TechnologyPantnagarIndia
  3. 3.Molecular Biology and BiotechnologyCSMCRIBhavnagarIndia