Applied Biochemistry and Biotechnology

, Volume 160, Issue 4, pp 1116–1123

Improving Enzymatic Production of Ginsenoside Rh2 from Rg3 by using Nonionic Surfactant

Article

DOI: 10.1007/s12010-009-8570-7

Cite this article as:
Su, J., Xu, J. & Wang, Z. Appl Biochem Biotechnol (2010) 160: 1116. doi:10.1007/s12010-009-8570-7
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Abstract

In this study, several nonionic surfactants were tried to improve the enzymatic hydrolysis of ginsenoside Rg3 into Rh2 which was catalyzed at 50 °C and pH 5.0 by a crude glucosidase extracted from Fusarium sp. ECU2042. Among the biocompatible nonionic surfactants, polyethylene glycol 350 monomethyl ether was shown to be the best. After optimizing some influencing factors on the reaction, the conversion of Rg3 (5 g/l) with 10 g/l crude enzyme reached almost 100% in the presence of the nonionic surfactant (7.5%, w/v), which was 25% higher than that in buffer without any surfactant. Furthermore, the enzyme stability was affected faintly by the surfactant.

Keywords

Nonionic surfactantFusarium sp. ECU2042BiohydrolysisGinsenoside Rg3Ginsenoside Rh2

Copyright information

© Humana Press 2009

Authors and Affiliations

  1. 1.Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  2. 2.School of PharmacyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China