Applied Biochemistry and Biotechnology

, Volume 158, Issue 1, pp 88–96

Influence of Silica-Derived Nano-Supporters on Cellobiase After Immobilization


DOI: 10.1007/s12010-008-8321-1

Cite this article as:
Wang, P., Hu, X., Cook, S. et al. Appl Biochem Biotechnol (2009) 158: 88. doi:10.1007/s12010-008-8321-1


Core shell magnetite nanoparticle (CSMN) was successfully synthesized with diameter around 125 nm according to the determination with scanning electronic microscopy. SBA-15 with diameter around 31 nm was synthesized in our previous work as another supporter for immobilized degradation enzymes. The aim of this study was to investigate the influence of silica-derived nano-supporters on cellobiase after immobilization. With covalent method, glutaraldehyde was introduced to immobilize cellobiase. The immobilized enzyme efficiency, specific activity, and its characterization, including optimum pH, pH stability, optimum temperature for enzyme reaction, and enzyme thermal stability were investigated. Results show that the method of enzyme immobilization on both nano-supporters could improve cellobiase stability under low pH and high temperature conditions compared with the free enzyme. In the aspect of immobilization efficiency, SBA had higher amount of bounded protein than that of CSMN, but had lower specific enzyme activity than CSMN, assumably due to the change in silica surface properties caused by process of supporter synthesis.


CellobiaseImmobilizationCore shell magnetite nanoparticleSBA

Copyright information

© Humana Press 2008

Authors and Affiliations

  • Peng Wang
    • 1
  • Xiaoke Hu
    • 1
  • Sean Cook
    • 1
  • Huey-Min Hwang
    • 1
  1. 1.Biology DepartmentJackson State UniversityJacksonUSA