Article

Applied Biochemistry and Biotechnology

, Volume 152, Issue 2, pp 199-212

First online:

Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate

  • Zhang Hou-RuiAffiliated withPhytochemical Department, Guangxi Institute of Botany, The Chinese Academy of Sciences Email author 
  • , Qin Xiang-XiangAffiliated withPhytochemical Department, Guangxi Institute of Botany, The Chinese Academy of Sciences
  • , Silvio S. SilvaAffiliated withBiotechnology Department, Engineering School of Lorena-São Paulo University
  • , Boutros F. SarrouhAffiliated withBiotechnology Department, Engineering School of Lorena-São Paulo University
  • , Cai Ai-HuaAffiliated withPhytochemical Department, Guangxi Institute of Botany, The Chinese Academy of Sciences
  • , Zhou Yu-HengAffiliated withPhytochemical Department, Guangxi Institute of Botany, The Chinese Academy of Sciences
  • , Jin KeAffiliated withPhytochemical Department, Guangxi Institute of Botany, The Chinese Academy of Sciences
  • , Xiang QiuAffiliated withBiotechnology Department, Gui Lin Medical College

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Abstract

In this paper, two new strians, Issatchenkia occidentalis (Lj-3, CCTCC M 2006097) and Issatchenkia orienalis (S-7, CCTCC M 2006098), isolated from different environments on solid media, were used in the detoxification process of the hemicellulosic hydrolysate of sugarcane bagasse. High-pressure liquid chromatography elution curve of UV-absorption compounds represented by acetic acid, furfural, and guaiacol (toxic compounds found in the hemicellulosic hydrolysate) showed that several chromatographic peaks were evidently diminished for the case of detoxified hydrolysate with isolate strains compared to the high peaks resulted for no detoxified hydrolysate. It was clear that these inhibitors were degraded by the two new isolates during their cultivation process. Fermentation results for the biodetoxified hydrolysate showed an increase in xylitol productivity (Q p) by 1.97 and 1.95 times (2.03 and 2.01 g l−1 h−1) and in xylitol yield (Y p) by 1.72 and 1.65 times (0.93 and 0.89 g xylitol per gram xylose) for hydrolysate treated with S-7 and Lj-3, respectively, in comparison with no detoxified hydrolysate (1.03 g l−1 h−1 and 0.54 g xylitol per gram xylose). This present work demonstrated the importance of Issatchenkia yeast in providing an effective biological detoxification approach to remove inhibitors and improve hydrolysate fermentability, leading to a high xylitol productivity and yield.

Keywords

Hemicellulosic hydrolysate Toxic compounds Biodetoxification Issatchenkia orientalis (S-7) Issatchenkia occidentalis (Lj-3) Xylitol