Applied Biochemistry and Biotechnology

, Volume 152, Issue 1, pp 29–41

Deactivation Kinetics and Response Surface Analysis of the Stability of α-l-Rhamnosidase from Penicillium decumbens


DOI: 10.1007/s12010-008-8204-5

Cite this article as:
Magario, I., Neumann, A., Oliveros, E. et al. Appl Biochem Biotechnol (2009) 152: 29. doi:10.1007/s12010-008-8204-5


The stability of the mixed enzyme preparation Naringinase from Penicillium decumbens was studied in dependence of the temperature, the pH value, and the enzyme concentration by means of response surface methodology. Deactivation kinetics by formation of an intermediate state was proposed for fitting deactivation data. Empirical models could then be constructed for prediction of deactivation rate constants, specific activity of intermediate state, and half-life values under different incubation conditions. From this study, it can be concluded that (1) Naringinase is most stable in the pH range of 4.5–5.0, being quite sensitive to lower pHs (<3.5) and (2) the glyco-enzyme is a rather thermo-stable enzyme preserving its initial activity for long times when incubated at its optimal pH up to temperatures of 65 °C. Enriched α-l-rhamnosidase after column treatment and ultrafiltration presented similar deactivation kinetics pattern and half-life values as the unpurified enzyme. Thus, any influence of low molecular weight substances on its deactivation is most probably negligible. The intermediate state of the enzyme may correspond to unfolding and self-digestion of its carbohydrate portion, lowering its activity relative to the initial state. The digestion- and unfolding-grade of this intermediate state may also be controlled by the pH and temperature of incubation.


Naringinase Doehlert array Response surface methodology Series-type deactivation kinetics α-Rhamnosidase Enzyme stability 

Copyright information

© Humana Press 2008

Authors and Affiliations

  • I. Magario
    • 1
  • A. Neumann
    • 1
  • E. Oliveros
    • 2
    • 3
  • C. Syldatk
    • 1
  1. 1.Institute of Engineering in Life Sciences, Chair of Technical BiologyUniversity of Karlsruhe (TH)KarlsruheGermany
  2. 2.Lehrstuhl für Umweltmesstechnik, Engler-Bunte-InstitutUniversität KarlsruheKarlsruheGermany
  3. 3.Laboratoire des IMRCP, UMR CNRS 5623Université Paul SabatierToulouse cédex 9France

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