Applied Biochemistry and Biotechnology

, Volume 126, Issue 1, pp 35–48

Macrophage-stimulating activity of exo-biopolymer from cultured rice bran with Monascus pilosus

  • K. W. Yu
  • Y. S. Kim
  • K. S. Shin
  • J. M. Kim
  • H. J. Suh
Original Articles

DOI: 10.1007/s12010-005-0004-6

Cite this article as:
Yu, K.W., Kim, Y.S., Shin, K.S. et al. Appl Biochem Biotechnol (2005) 126: 35. doi:10.1007/s12010-005-0004-6
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Abstract

To find a new use of rice bran, five fungi were examined for the production of exo-biopolymer with macrophage-stimulating activity from rice bran. Among the exo-biopolymers produced from the cultures, Monascus pilosus had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve the yield of exo-biopolymer with macrophage-stimulating activity, a suitable medium for exo-biopolymer was tested in submerged culture of M. pilosus. The highest amount of exo-biopolymer (13.9 mg/mL) was obtained in a medium containing rice bran as an only carbon source followed by media with additional maltose and sucrose (13.8 and 13.7 mg/mL, respectively). The addition of peptone resulted in the production of high amount of exo-biopolymer (15.1 mg/mL), meanwhile the addition of ammonium chloride resulted in 264.0 µg/mL of glucosamine content. Among eight different kinds of inorganic salts tested, potassium phosphate (0.1%) was the most effective inorganic salt for the mycelial growth and exo-biopolymer production. Therefore the optimal medium composition was as follows (g/L): 20 g of rice bran, 5 g of peptone, and 1 g of KH2PO4. The optimal culture pH and time for mycelial growth and exo-biopolymer production was pH 5.0 and 25°C, respectively. The maximum exo-biopolymer (20.1 mg/mL) was observed at the fourth day of cultivation. Exo-biopolymer, a crude polysaccharide fraction, mainly contained neutral sugar (81.8%) with considerable amounts of uronic acid (18.2%). Component sugar analysis showed that the active fraction consisted mainly of arabinose, galactose, glucose, which was digested from starch of rice bran during cultivation, and uronic acid (molar ratio; 0.8:1.0:0.7:0.8).

Index Entries

Macrophage-stimulating activityMonascus pilosusrice bransubmerged culture

Copyright information

© Humana Press Inc 2005

Authors and Affiliations

  • K. W. Yu
    • 1
  • Y. S. Kim
    • 2
  • K. S. Shin
    • 3
  • J. M. Kim
    • 4
  • H. J. Suh
    • 5
  1. 1.Department of Kimchi and Food ScienceChongju National College of Science and TechnologyJeungpyeongKorea
  2. 2.Korea Food Research InstituteSungnamKorea
  3. 3.Department of Food Science and BiotechnologyKyonggi UniversitySuwonKorea
  4. 4.Department of Food Science and Biotechnology of Animal ResourcesKonkuk UniversitySeoulKorea
  5. 5.Department of Food and Nutrition, College of Heath SciencesKorea UniversitySeoulKorea