Clinical Orthopaedics and Related Research®

, Volume 468, Issue 10, pp 2725–2733

Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential: A Pilot Study

  • Tomokazu Yoshida
  • Masakazu Ishikawa
  • Yuji Yasunaga
  • Takuma Yamasaki
  • Mitsuo Ochi
Basic Research

DOI: 10.1007/s11999-010-1444-z

Cite this article as:
Yoshida, T., Ishikawa, M., Yasunaga, Y. et al. Clin Orthop Relat Res (2010) 468: 2725. doi:10.1007/s11999-010-1444-z
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Abstract

Background

Cell therapy using autologous cells has been used in the treatment of various medical conditions. The mononuclear cell (MNC) fraction of bone marrow (BM) contains stem/progenitor cells that could contribute to osteogenesis and angiogenesis.

Questions/purposes

We asked whether MNCs derived from intraoperative shed blood (SB), consisting of peripheral blood and BM, have osteoinductive and angiogenic potential.

Methods

We harvested SB and BM from six patients undergoing THA. Isolated MNCs from SB and BM were analyzed by flow cytometry to evaluate the CD34+ cell fraction and 1 × 106 cells were seeded on an interconnective porous calcium hydroxyapatite ceramic (IP-CHA) and transplanted in the backs of athymic rats. IP-CHAs without cells were transplanted as controls and all composites were harvested after 4 and 8 weeks. Osteoinductive potential was evaluated by histologic observation, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) using anti-osteocalcin (OC) antibodies qualitatively and quantitatively. To evaluate angiogenic potential, capillary density was measured by immunohistochemistry using Isolectin B4 4 weeks after implantation.

Results

We found that CD34+ cells existed in SB-MNCs and there was a trend toward lower frequency compared with BM-MNCs. Histologic osteoinduction, OC expression, and capillary density were increased by transplantation of MNCs from SB. Similar results were achieved with MNCs from BM.

Conclusions

MNCs from SB have equivalent osteoinductive and angiogenic potential compared with those from BM.

Clinical Relevance

SB could be an attractive source for isolation of MNCs, enhancing osteoinduction and neovascularization, to augment the reconstruction of skeletal defects.

Copyright information

© The Association of Bone and Joint Surgeons® 2010

Authors and Affiliations

  • Tomokazu Yoshida
    • 1
  • Masakazu Ishikawa
    • 1
  • Yuji Yasunaga
    • 2
  • Takuma Yamasaki
    • 1
  • Mitsuo Ochi
    • 1
  1. 1.Department of Orthopaedic Surgery, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan
  2. 2.Department of Artificial Joints and Biomaterials, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan