Different Differentiation of Stroma Cells from Patients with Osteonecrosis: A Pilot Study
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Osteonecrosis (ON) is commonly caused by high doses of glucocorticoids or ethanol intake. We previously reported suppression of BMP-2 by dexamethasone was more pronounced and enhancement by lovastatin was less pronounced in bone marrow stromal cells (BMSCs) from patients with ON than from patients without ON. We therefore presumed the BMSCs might be dysfunctional in patients with ON and performed this pilot study. We obtained BMSCs from 10 patients with ethanol-induced ON, 10 patients with glucocorticoid-induced ON, and 12 patients without ON as control subjects, checking third passage cells for osteogenic and adipogenic gene expression and differentiation ability. BMSCs from patients with glucocorticoid-induced ON possessed less osteogenic gene expression and less osteogenic differentiation, whereas BMSCs from patients with ethanol-induced ON possessed more adipogenic gene expression and more adipogenic differentiation. Dysfunction of BMSCs may be one of the causes of ON, with differing dysfunction in ethanol-induced ON and glucocorticoid-induced ON. Glucocorticoids may possess more of a suppressive effect on osteogenesis than ethanol, whereas ethanol may possess a more potent adipogenic effect than glucocorticoids on BMSCs.
- Arlet J. Nontraumatic avascular necrosis of the femoral head: past, present, and future. Clin Orthop Relat Res. 1992;277:12–21.
- Chang JK, Ho ML, Yeh CH, Chen CH, Wang GJ. Osteogenic gene expression decreases in stromal cells of patients with osteonecrosis. Clin Orthop Relat Res. 2006;453:286–292. CrossRef
- Chang JK, Li CJ, Wu SC, Yeh CH, Chen CH, Fu YC, Wang GJ, Ho ML. Effects of anti-inflammatory drugs on proliferation, cytotoxicity and osteogenesis in bone marrow mesenchymal stem cells. Biochem Pharmacol. 2007;74:1371–1382. CrossRef
- Chen CH, Ho ML, Chang JK, Hung SH, Wang GJ. Green tea catechin enhances osteogenesis in a bone marrow mesenchymal stem cell line. Osteoporos Int. 2005;16:2039–2045. CrossRef
- Cui Q, Wang GJ, Balian G. Steroid-induced adipogenesis in a pluripotential cell line from bone marrow. J Bone Joint Surg Am. 1997;79:1054–1063.
- Cui Q, Wang GJ, Balian G. Pluripotential marrow cells produce adipocytes when transplanted into steroid-treated mice. Connect Tissue Res. 2000;41:45–56. CrossRef
- Cui Q, Wang GJ, Su CC, Balian G. The Otto Aufranc Award. Lovastatin prevents steroid induced adipogenesis and osteonecrosis. Clin Orthop Relat Res. 1997;344:8–19. CrossRef
- Diduch DR, Coe MR, Joyner C, Owen ME, Balian G. Two cell lines from bone marrow that differ in terms of collagen synthesis, osteogenic characteristics, and matrix mineralization. J Bone Joint Surg Am. 1993;75:92–105.
- Felson DT, Anderson JJ. A cross-study evaluation of association between steroid dose and bolus steroids and avascular necrosis of bone. Lancet. 1987;1:902–906. CrossRef
- Gohel A, McCarthy MB, Gronowicz G. Estrogen prevents glucocorticoid-induced apoptosis in osteoblasts in vivo and in vitro. Endocrinology. 1999;140:5339–5347. CrossRef
- Hung SH, Yeh CH, Huang HT, Wu P, Ho ML, Chen CH, Wang C, Chao D, Wang GJ. Pioglitazone and dexamethasone induce adipogenesis in D1 bone marrow stromal cell line, but not through the peroxisome proliferator-activated receptor-gamma pathway. Life Sci. 2008;82:561–569. CrossRef
- Jones JP Jr, Engleman EP, Najarian JS. Systemic fat embolism after renal homotransplantation and treatment with corticosteroids. N Engl J Med. 1965;273:1453–1458.
- Jones JP Jr, Ramirez S, Doty SB. The pathophysiologic role of fat in dysbaric osteonecrosis. Clin Orthop Relat Res. 1993;296:256–264.
- Kawai K, Tamaki A, Hirohata K. Steroid-induced accumulation of lipid in the osteocytes of the rabbit femoral head: a histochemical and electron microscopic study. J Bone Joint Surg Am. 1985;67:755–763.
- Kitajima M, Shigematsu M, Ogawa K, Sugihara H, Hotokebuchi T. Effects of glucocorticoid on adipocyte size in human bone marrow. Med Mol Morphol. 2007;40:150–156. CrossRef
- Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood. 2004;103:1669–1675. CrossRef
- Lin RW, Chen CH, Wang YH, Ho ML, Hung SH, Chen IS, Wang GJ. (-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-kappaB. Biochem Biophys Res Commun. 2009;379:1033–1037. CrossRef
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25:402–408. CrossRef
- Mankin HJ. Nontraumatic necrosis of bone (osteonecrosis). N Engl J Med. 1992;326:1473–1479.
- Puchtler H, Meloan SN, Terry MS. On the history and mechanism of alizarin and alizarin red S stains for calcium. J Histochem Cytochem. 1969;17:110–124.
- Sheng HH, Zhang GG, Cheung WH, Chan CW, Wang YX, Lee KM, Wang HF, Leung KS, Qin LL. Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development. J Orthop Surg. 2007;2:15. CrossRef
- Wang GJ, Cui Q, Balian G. The Nicolas Andry Award. The pathogenesis and prevention of steroid-induced osteonecrosis. Clin Orthop Relat Res. 2000;370:295–310.
- Wang GJ, Hubbard SL, Reger SI, Miller ED, Stamp WG. Femoral head blood flow in long-term steroid therapy: study of rabbit model. South Med J. 1983;76:1530–1532.
- Wang GJ, Sweet DE, Reger SI, Thompson RC. Fat-cell changes as a mechanism of avascular necrosis of the femoral head in cortisone-treated rabbits. J Bone Joint Surg Am. 1977;59:729–735.
- Wang Y, Li Y, Mao K, Li J, Cui Q, Wang GJ. Alcohol-induced adipogenesis in bone and marrow: a possible mechanism for osteonecrosis. Clin Orthop Relat Res. 2003;410:213–224. CrossRef
- Yeh CH, Chang JK, Wang YH, Ho ML, Wang GJ. Ethanol may suppress Wnt/beta-catenin signaling on human bone marrow stroma cells: a preliminary study. Clin Orthop Relat Res. 2008;466:1047–1053. CrossRef
- Different Differentiation of Stroma Cells from Patients with Osteonecrosis: A Pilot Study
Clinical Orthopaedics and Related Research®
Volume 467, Issue 8 , pp 2159-2167
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Department of Physiology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
- 2. Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan
- 3. Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
- 4. Center for Cervical Cancer Prevention, Department of Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
- 5. Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
- 6. Department of Orthopaedics, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
- 7. Department of Orthopaedics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 100 Tzu-You 1st Road, Kaohsiung City, Taiwan
- 8. Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA
- 9. Department of Orthopaedic Surgery, National Cheng-Kung University, No 100 Shi-Chuan 1st Road, Kaohsiung City, Taiwan