, Volume 467, Issue 8, pp 2159-2167
Date: 28 Mar 2009

Different Differentiation of Stroma Cells from Patients with Osteonecrosis: A Pilot Study

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Osteonecrosis (ON) is commonly caused by high doses of glucocorticoids or ethanol intake. We previously reported suppression of BMP-2 by dexamethasone was more pronounced and enhancement by lovastatin was less pronounced in bone marrow stromal cells (BMSCs) from patients with ON than from patients without ON. We therefore presumed the BMSCs might be dysfunctional in patients with ON and performed this pilot study. We obtained BMSCs from 10 patients with ethanol-induced ON, 10 patients with glucocorticoid-induced ON, and 12 patients without ON as control subjects, checking third passage cells for osteogenic and adipogenic gene expression and differentiation ability. BMSCs from patients with glucocorticoid-induced ON possessed less osteogenic gene expression and less osteogenic differentiation, whereas BMSCs from patients with ethanol-induced ON possessed more adipogenic gene expression and more adipogenic differentiation. Dysfunction of BMSCs may be one of the causes of ON, with differing dysfunction in ethanol-induced ON and glucocorticoid-induced ON. Glucocorticoids may possess more of a suppressive effect on osteogenesis than ethanol, whereas ethanol may possess a more potent adipogenic effect than glucocorticoids on BMSCs.

One or more of the authors (JKC, MLH, CHC, GJW) have received funding from the National Health Research Institute of Taiwan (NHRI-EX94-9316EP and NHRI-EX96-9615EP), Kaohsiung Medical University Hospital (93-KMUH-019), and the Technology Development Program for Academia in Taiwan (96-EC-17-A-17-S1-041).
Each author certifies that his or her institution has approved for the human protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.
This work was performed at Kaohsiung Medical University Hospital.