Clinical Orthopaedics and Related Research

, Volume 467, Issue 1, pp 273–280

Effects of a New Allograft Processing Procedure on Graft Healing in a Canine Model: A Preliminary Study

  • Kathleen S. Beebe
  • Joseph Benevenia
  • Benjamin E. Tuy
  • C. Alex DePaula
  • Robert D. Harten
  • William F. Enneking
Original Article

DOI: 10.1007/s11999-008-0444-8

Cite this article as:
Beebe, K.S., Benevenia, J., Tuy, B.E. et al. Clin Orthop Relat Res (2009) 467: 273. doi:10.1007/s11999-008-0444-8
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Abstract

Graft healing in vivo can be affected by allograft processing. We asked whether a new processing technique influenced graft-host healing compared with autograft and a standard processing technique in a canine ulna model. We used bilateral intercalary allografts or autografts in the ulna of 13 skeletally mature male coonhounds. Each animal received two allografts, either one autograft and one allograft, or two autografts. At term (90 days), the graft sites were harvested. We assessed union with high-resolution xray imaging. Each specimen was processed for nondecalcified histologic analysis to assess the graft-host interface. Quantitative histomorphometric analysis was performed to determine spatial location and area of bone. Radiographic analysis, histologic analysis, and histomorphometric measures revealed no differences in union, mean total bone area, or total endosteal/intramedullary bone for the new process, standard process, and autografts. Our preliminary data suggest the new processing techniques may increase the safety of allograft transplantation without adversely affecting union when compared with standard processing techniques and autograft in a canine model.

Copyright information

© The Association of Bone and Joint Surgeons 2008

Authors and Affiliations

  • Kathleen S. Beebe
    • 1
  • Joseph Benevenia
    • 1
  • Benjamin E. Tuy
    • 1
  • C. Alex DePaula
    • 2
  • Robert D. Harten
    • 3
  • William F. Enneking
    • 4
  1. 1.Department of OrthopaedicsUMDNJ-New Jersey Medical SchoolNewarkUSA
  2. 2.Synthes (USA)West ChesterUSA
  3. 3.Synthes (USA)PaoliUSA
  4. 4.Department of Orthopaedics and RehabilitationUniversity of FloridaGainesvilleUSA

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