Original Paper

Food and Bioprocess Technology

, Volume 4, Issue 6, pp 1066-1088

First online:

Native and Biotechnologically Engineered Plant Proteases with Industrial Applications

  • Lucía Feijoo-SiotaAffiliated withDepartment of Microbiology, Faculty of Pharmacy and School of Biotechnology, University of Santiago de Compostela
  • , Tomás G. VillaAffiliated withDepartment of Microbiology, Faculty of Pharmacy and School of Biotechnology, University of Santiago de Compostela Email author 

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Abstract

Proteases occupy the most relevant position among industrial enzymes. Plant proteases have been used in medicine, detergent manufacturing, and food science for many years, but their production is diminishing in favor of those of microbial origin because lower production costs. Papain, bromelain, and ficin are the most frequently employed plant proteases, although new proteases with new and more appealing physicochemical properties for industry are still emerging. DNA technology and genetic engineering shall play, without a doubt, an important role for the production of these proteases at the industrial level. The present review focuses on the applications of traditional plant proteases as well as new proteases discovered during the last 20 years, some of which have already been genetically engineered either to increase production or to strengthen some of their physicochemical properties. The review also refers to the protease classification, action pattern, and main characteristics.

Keywords

Plant proteases Industrial enzymes Protein engineering Genetic engineering Applications