Food and Bioprocess Technology

, Volume 5, Issue 2, pp 696–705

Optimization of Enzymatic Hydrolysis of Visceral Waste Proteins of Yellowfin Tuna (Thunnus albacares)

  • Mahmoudreza Ovissipour
  • Abdolmohammad Abedian Kenari
  • Ali Motamedzadegan
  • Rajab Mohammad Nazari
Original Paper

DOI: 10.1007/s11947-010-0357-x

Cite this article as:
Ovissipour, M., Abedian Kenari, A., Motamedzadegan, A. et al. Food Bioprocess Technol (2012) 5: 696. doi:10.1007/s11947-010-0357-x

Abstract

Fish protein hydrolysate was produced from the viscera of yellowfin tuna (Thunnus albacares). Hydrolysis conditions (enzyme activity, temperature, and time) were optimized using response surface methodology. A factorial design was applied to minimize enzyme utilization and modeling of degree of hydrolysis (r2 = 0.94). Lack-of-fit test revealed a non-significant value for the model, indicating that the regression equation was adequate for predicting the degree of hydrolysis under any combination of the variables (P < 0.05). The optimum conditions to reach the highest degree of hydrolysis were: 60.4 °C, 90.25 min, and a protease (Alcalase 2.4 L) activity of 70.22 AU/kg protein. The spray-dried tuna visceral protein hydrolysates had relatively high protein (72.34%) and low lipid (1.43%) content. The chemical score of the hydrolysate indicated that it fulfils adult human nutritional requirements except for methionine. Lysine and methionine were the first and the second limiting amino acids in that order. Phenylalanine was the predominant amino acid in the hydrolysates with respect to common carp requirement. In addition, the protein efficiency ratio of tuna visceral hydrolysate was 2.85–5.35.

Keywords

Fish protein hydrolysates Tuna visceral protein Alcalase Optimization RSM 

Copyright information

© Springer Science + Business Media, LLC 2010

Authors and Affiliations

  • Mahmoudreza Ovissipour
    • 1
  • Abdolmohammad Abedian Kenari
    • 1
  • Ali Motamedzadegan
    • 2
  • Rajab Mohammad Nazari
    • 3
  1. 1.Department of Fisheries, Faculty of Natural Resources and Marine SciencesTarbiat Modares UniversityNoorIran
  2. 2.Department of Food ScienceSari Agricultural Sciences and Natural Resources UniversitySariIran
  3. 3.Shahid Rajaee Sturgeon Hatchery CenterSariIran

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