Plant Biotechnology Reports

, Volume 2, Issue 4, pp 239–243

ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis)

Authors

  • Kuldeep Sharma
    • Department of BotanyUniversity of Delhi
    • Department of BotanyUniversity of Delhi
  • Sarika Gupta
    • National Institute for Plant Genome Research (NIPGR)
  • Ravindra Kumar
    • Department of BotanyUniversity of Delhi
  • Manoj Prasad
    • National Institute for Plant Genome Research (NIPGR)
Original Article

DOI: 10.1007/s11816-008-0070-7

Cite this article as:
Sharma, K., Agrawal, V., Gupta, S. et al. Plant Biotechnol Rep (2008) 2: 239. doi:10.1007/s11816-008-0070-7

Abstract

Simmondsia chinensis (Link) Schneider, a multipurpose and monogeneric dioecious shrub from arid zones, has emerged as a cash crop all over the globe. Its seed propagation poses severe problems due to its male-biased population: the male:female ratio is 5:1. Investigations have been carried out to generate a sex-specific Inter-simple sequence repeat (ISSR) marker for the early detection of male and female plants. Of the 42 primers analysed with a bulk sample of pooled male DNA and a bulk sample of pooled female DNA, only one primer, UBC-807, produced a unique ~1,200 base-pair fragment in the male DNA. To validate this observation, this primer was re-tested with individual male and female samples from eight cultivars. A similar unique ~1,200 bp fragment was present in the male individuals of all eight cultivars and completely absent in the female individuals tested. This is the first report of the use of ISSR markers to ascertain sex in physiologically mature S. chinensis plants.

Keywords

DioeciousISSR markerSimmondsiachinensis

Copyright information

© Korean Society for Plant Biotechnology and Springer 2008