, Volume 1, Issue 4, pp 251-257

Induction of differential flower pigmentation patterns by RNAi using promoters with distinct tissue-specific activity

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

We attempted to evaluate the effects of promoters on flower color modification by RNA interference (RNAi). An inverted repeat of a tobacco chalcone synthase (CHS) fragment was used as a trigger under control of three different promoters and transformed into tobacco plants. The flowers of CaMV35S:CHSir displayed a completely white color, whereas those of Agrobacterium rhizogenes rolC:CHSir kept slight pigmentation. On the other hand, gentian GtCHS:CHSir showed decreased pigmentation in the tip only but not at the base of the petal limbs, which resulted in the appearance of bicolor-like flowers. The observed suppression of flower pigmentation corresponded with tissue-specificity of each promoter activity determined by histochemical GUS assay using transgenic tobacco plants with each promoter–GUS construct. These results demonstrate for the first time that RNAi-mediated silencing can be used to produce novel-colored flowers with high horticultural value.