Plant Biotechnology Reports

, Volume 1, Issue 4, pp 251–257

Induction of differential flower pigmentation patterns by RNAi using promoters with distinct tissue-specific activity


  • Takashi Nakatsuka
    • Iwate Biotechnology Research Center
  • Chetsadaporn Pitaksutheepong
    • National Center for Genetic Engineering and Biotechnology
  • Saburo Yamamura
    • Iwate Biotechnology Research Center
    • Iwate Biotechnology Research Center
Original Article

DOI: 10.1007/s11816-007-0039-y

Cite this article as:
Nakatsuka, T., Pitaksutheepong, C., Yamamura, S. et al. Plant Biotechnol Rep (2007) 1: 251. doi:10.1007/s11816-007-0039-y


We attempted to evaluate the effects of promoters on flower color modification by RNA interference (RNAi). An inverted repeat of a tobacco chalcone synthase (CHS) fragment was used as a trigger under control of three different promoters and transformed into tobacco plants. The flowers of CaMV35S:CHSir displayed a completely white color, whereas those of Agrobacterium rhizogenesrolC:CHSir kept slight pigmentation. On the other hand, gentian GtCHS:CHSir showed decreased pigmentation in the tip only but not at the base of the petal limbs, which resulted in the appearance of bicolor-like flowers. The observed suppression of flower pigmentation corresponded with tissue-specificity of each promoter activity determined by histochemical GUS assay using transgenic tobacco plants with each promoter–GUS construct. These results demonstrate for the first time that RNAi-mediated silencing can be used to produce novel-colored flowers with high horticultural value.


AnthocyaninBicolorFlower color patternRNAiTissue-specific promoterTobacco



Cauliflower mosaic virus


Chalcone synthase


Double-stranded RNA


RNA interference


Small interference RNA

Copyright information

© Korean Society for Plant Biotechnology and Springer 2007