Rapid Communication

Korean Journal of Chemical Engineering

, Volume 29, Issue 12, pp 1666-1669

First online:

Use of biologically designed gold nanowire for biosensor application

  • Sung-Hee ShinAffiliated withSchool of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)
  • , Gha-Young KimAffiliated withDepartment of Nuclear Fuel Cycle Technology Development, Korea Atomic Energy Research Institute
  • , Joonmok ShimAffiliated withEnergy Storage Center, Korea Institute of Energy Research (KIER)
  • , Jungok KimAffiliated withSchool of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)
  • , Hor-Gil HurAffiliated withSchool of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)
  • , Don-Jung LeeAffiliated withDepartment of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST)
  • , Jong-In SongAffiliated withDepartment of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST)
  • , Seung-Hyeon MoonAffiliated withSchool of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST) Email author 

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Abstract

A highly sensitive tyrosinase (TYR)-based amperometric biosensor is prepared using biologically designed gold nanowires (AuNWs) for pesticide detection. The AuNWs were synthesized by dodecapeptide Midas-11 and were modified with the formation of self-assembled monolayer (SAM), followed by covalent binding with TYR. The prepared TYR-AuNWs-SPCE (screen printed carbon electrode) was compared with bare, AuNWs-, modified-AuNWs-SPCE by the measurement of cyclic voltammetry. The quantitative relationship between the inhibition percentage and the pesticide concentration at the TYR-AuNWs-SPCE was obtained by measuring the current response in various concentrations of pesticides. The reasonable detection range of parathion was determined to be 0.1 ppt through 10 ppb (R 2 =0.990) with 0.087 ppt of detection limits. The higher sensitivity and wider detection range of the TYR-based biosensor was achieved by the use of biologically synthesized AuNWs.

Key words

Tyrosinase Gold Nanowire Peptide Amperometric Biosensor Pesticide