Korean Journal of Chemical Engineering

, Volume 29, Issue 12, pp 1666–1669

Use of biologically designed gold nanowire for biosensor application

Authors

  • Sung-Hee Shin
    • School of Environmental Science and EngineeringGwangju Institute of Science and Technology (GIST)
  • Gha-Young Kim
    • Department of Nuclear Fuel Cycle Technology DevelopmentKorea Atomic Energy Research Institute
  • Joonmok Shim
    • Energy Storage CenterKorea Institute of Energy Research (KIER)
  • Jungok Kim
    • School of Environmental Science and EngineeringGwangju Institute of Science and Technology (GIST)
  • Hor-Gil Hur
    • School of Environmental Science and EngineeringGwangju Institute of Science and Technology (GIST)
  • Don-Jung Lee
    • Department of Nanobio Materials and ElectronicsGwangju Institute of Science and Technology (GIST)
  • Jong-In Song
    • Department of Nanobio Materials and ElectronicsGwangju Institute of Science and Technology (GIST)
    • School of Environmental Science and EngineeringGwangju Institute of Science and Technology (GIST)
Rapid Communication

DOI: 10.1007/s11814-012-0140-y

Cite this article as:
Shin, S., Kim, G., Shim, J. et al. Korean J. Chem. Eng. (2012) 29: 1666. doi:10.1007/s11814-012-0140-y

Abstract

A highly sensitive tyrosinase (TYR)-based amperometric biosensor is prepared using biologically designed gold nanowires (AuNWs) for pesticide detection. The AuNWs were synthesized by dodecapeptide Midas-11 and were modified with the formation of self-assembled monolayer (SAM), followed by covalent binding with TYR. The prepared TYR-AuNWs-SPCE (screen printed carbon electrode) was compared with bare, AuNWs-, modified-AuNWs-SPCE by the measurement of cyclic voltammetry. The quantitative relationship between the inhibition percentage and the pesticide concentration at the TYR-AuNWs-SPCE was obtained by measuring the current response in various concentrations of pesticides. The reasonable detection range of parathion was determined to be 0.1 ppt through 10 ppb (R2=0.990) with 0.087 ppt of detection limits. The higher sensitivity and wider detection range of the TYR-based biosensor was achieved by the use of biologically synthesized AuNWs.

Key words

TyrosinaseGold NanowirePeptideAmperometric BiosensorPesticide

Copyright information

© Korean Institute of Chemical Engineers, Seoul, Korea 2012