Korean Journal of Chemical Engineering

, Volume 29, Issue 12, pp 1666–1669

Use of biologically designed gold nanowire for biosensor application

  • Sung-Hee Shin
  • Gha-Young Kim
  • Joonmok Shim
  • Jungok Kim
  • Hor-Gil Hur
  • Don-Jung Lee
  • Jong-In Song
  • Seung-Hyeon Moon
Rapid Communication

DOI: 10.1007/s11814-012-0140-y

Cite this article as:
Shin, SH., Kim, GY., Shim, J. et al. Korean J. Chem. Eng. (2012) 29: 1666. doi:10.1007/s11814-012-0140-y

Abstract

A highly sensitive tyrosinase (TYR)-based amperometric biosensor is prepared using biologically designed gold nanowires (AuNWs) for pesticide detection. The AuNWs were synthesized by dodecapeptide Midas-11 and were modified with the formation of self-assembled monolayer (SAM), followed by covalent binding with TYR. The prepared TYR-AuNWs-SPCE (screen printed carbon electrode) was compared with bare, AuNWs-, modified-AuNWs-SPCE by the measurement of cyclic voltammetry. The quantitative relationship between the inhibition percentage and the pesticide concentration at the TYR-AuNWs-SPCE was obtained by measuring the current response in various concentrations of pesticides. The reasonable detection range of parathion was determined to be 0.1 ppt through 10 ppb (R2=0.990) with 0.087 ppt of detection limits. The higher sensitivity and wider detection range of the TYR-based biosensor was achieved by the use of biologically synthesized AuNWs.

Key words

TyrosinaseGold NanowirePeptideAmperometric BiosensorPesticide

Copyright information

© Korean Institute of Chemical Engineers, Seoul, Korea 2012

Authors and Affiliations

  • Sung-Hee Shin
    • 1
  • Gha-Young Kim
    • 2
  • Joonmok Shim
    • 3
  • Jungok Kim
    • 1
  • Hor-Gil Hur
    • 1
  • Don-Jung Lee
    • 4
  • Jong-In Song
    • 4
  • Seung-Hyeon Moon
    • 1
  1. 1.School of Environmental Science and EngineeringGwangju Institute of Science and Technology (GIST)GwangjuKorea
  2. 2.Department of Nuclear Fuel Cycle Technology DevelopmentKorea Atomic Energy Research InstituteDaejeonKorea
  3. 3.Energy Storage CenterKorea Institute of Energy Research (KIER)DaejeonKorea
  4. 4.Department of Nanobio Materials and ElectronicsGwangju Institute of Science and Technology (GIST)GwangjuKorea