Carbon dioxide sequestration activity was compared and evaluated using bovine carbonic anhydrase (BCA), and a water soluble protein extract derived from hemocytes from diseased shell (HDS). Para-nitrophenyl acetate (p-NPA) was used to measure the reaction rate. The kcat/Km values obtained from the Lineweaver-Burk and Michaelis-Menten equations were 230.7M−1s−1 for BCA and 194.1M−s− for HDS. Without a biocatalyst, CaCO3 production took 15 seconds on average, while it took 5 seconds on average when BCA or HDS were present, indicating an approximately 3-fold enhancement of CaCO3 production rate by the biocatalysts. The biocatalytic hydration of CO2 and its precipitation as CaCO3 in the presence of biocatalysts were investigated.
Carbon DioxideSequestrationBovine Carbonic AnhydraseHemocytes from Diseased ShellBiomineralization