Journal of the American Oil Chemists' Society

, Volume 75, Issue 3, pp 405–410

Structured lipids: Lipase-catalyzed interesterification of tricaproin and trilinolein

Article

DOI: 10.1007/s11746-998-0059-y

Cite this article as:
Fomuso, L.B. & Akoh, C.C. J Amer Oil Chem Soc (1998) 75: 405. doi:10.1007/s11746-998-0059-y

Abstract

Structured lipids were synthesized by interesterification of trilinolein and tricaproin with sn-1,3-specific (IM 60) and nonspecific (SP 435) lipases. The interesterification reaction was performed by incubating a 1:2 mole ratio of trilinolein and tricaproin in 3 mL hexane at 45°C for the IM 60 lipase from Rhizomucor miehei, and at 55°C for the SP 435 lipase from Candida antarctica. Reaction products were analyzed by reverse-phase high-performance liquid chromatography with an evaporative light-scattering detector. The fatty acids at the sn-2 position were identified after pancreatic lipase hydrolysis and analysis with a gas chromatograph. IM 60 lipase produced 53,5 mol% dicaproyllinolein (total carbon number = C33) and 22.2% monocaproyldilinolein (C45). SP 435 lipase produced 41% C33 and 18% C45. When caproic acid was used in place of tricaproin as the acyl donor, the IM 60 lipase produced 62.9% C33. The effects of variation in mole ratio, temperature, added water, solvent polarity, and time course on the interesterification reaction were also investigated. In the absence of organic solvent, IM 60 lipase produced 52.3% C33.

Key Words

Candida antarcticainteresterificationlipaseorganic solventRhizomucor mieheistructured lipid

Copyright information

© AOCS Press 1998

Authors and Affiliations

  1. 1.Department of Food Science and TechnologyThe University of GeorgiaAthens