Abstract
Four bean varieties with different hull colors (white, brown, red, and black) were extracted with 80% acetone and evaluated for their antioxidant potential. Red, brown, and black bean extracts were able to effectively retain supercoiled DNA against 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced radical damage at the concentration levels (5, 10, 50, and 100 ppm) tested. Delphinidin, cyanidin, and procyanidins B2, C1, C2, and X were identified in red, brown, and black bean hull extracts, whereas white bean hull extract contained only a small amount of flavonoids. Vanillic, caffeic, p-coumaric, ferulic, and sinapic acids were the main phenolic acids identified in bean hull extracts.
Similar content being viewed by others
References
Wittenberg, L.W., Inhibition of Carcinogenesis by Minor Dietary Constituents, Cancer Res. 52 (Suppl.):2085S-2091S (1992).
Rizkalla, S.W., F. Bellisle, and G. Slama, Health Benefits of Low Glycemic Index Foods, Such as Pulses, in Diabetic Patients and Healthy Individuals, Br. J. Nutr. 88 (Suppl.3):S255-S262 (2002).
Champ, M.M.J., Non-nutrient Bioactive Substances of Pulses, Br. J. Nutr. 88 (Suppl. 3):S307-S319 (2002).
Mathers, J.C., Pulses and Carcinogenesis: Potential for the Prevention of Colon, Breast and Other Cancers, Br. J. Nutr. 88, (Suppl. 3):S273-S279 (2002).
Singh, D.K., and S.M. Lippmann, Cancer Chemoprevention Part 1: Retinoids and Carotenoids and Other Classic Antioxidants, Oncology 12:1643–1682 (1998).
Shahidi, F., U.D. Chavan, M. Naczk, and R. Amarowicz, Nutrient Distribution and Phenolic Antioxidants in Air Classified Fractions of Beach Pea (Lathyrus maritimus L.), J. Agric. Food Chem. 49:926–933 (2001).
Singleton, V.L., and J.A. Rossi, Colorimetry of Total Phenolics with Phosphomolybdic Phosphotungstic Acid Reagents, Am. J. Enol. Vitic. 16:144–158 (1965).
Hu, C., Y. Zhang, and D.D. Kitts, Evaluation of Antioxidant and Prooxidant Activities of Bamboo Phyllostachys nigra Var. Henonis Leaf Extract in vitro, J. Agric. Food Chem. 48:3170–3176 (2000).
Porter, L.J., L.N. Hrstich and B.G. Chan, The Conversion of Procyanidins and Delphinidins to Cyanidin and Delphindin, Phytochemistry 25:223–230 (1986).
Hong, V., and R. Wrolstad, Characterization of Anthocyanin-Containing Colorants and Fruit Juices by HPLC/Photodiode Array Detection, J. Am. Oil Chem. Soc. 38:698–708 (1990).
Oszmianski, J., and M. Moutounet, Color Product of Reaction Between Malvinidin Glucoside, Flavonols and Acetaldehyde, Pol. J. Food Nutr. Sci. 463:73–80 (1996).
Amarowicz, R., and S. Weidner, Content of Phenolic Acids in Rye Caryopses Determined Using HPLC/Photodiode Array Detection, Czech. J. Food Sci. 19:210–215 (2001).
Amarowicz, R., and F. Shahidi, Chromatographic Separation of Glucopyranyl Sinapate from Canola Meal, J. Am. Oil Chem. Soc. 71:551–560 (1994).
Breimer, L.H., Molecular Mechanisms of Oxygen Radical Carcinogenesis and Mutagenesis: The Role of DNA Base Damage, Mol. Carcinog. 3:188–197 (1990).
Ross, A.B., and B.H.J. Beilski, Sources of Compilations of Rate Constants for Oxygen Radicals in Solution, Methods Enzymol. 186:113–116 (1990).
Morrero, R., and L.J. Marnett, The Role of Organic Peroxy Radicals in Carcinogenesis, in DNA and Free Radicals, edited by B. Halliwell and O.I. Aruoma, Ellis Horwood, New York, 1993, pp. 145–146.
Rice-Evans, C., N. Miller, and G. Paganga, Structure-Antioxidant Relationship of Flavonoids and Phenolic Acids, Free Rad. Biol. Med. 20:933–956 (1996).
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Madhujith, T., Amarowicz, R. & Shahidi, F. Phenolic antioxidants in beans and their effects on inhibition of radical-induced DNA damage. J Amer Oil Chem Soc 81, 691–696 (2004). https://doi.org/10.1007/s11746-004-963-y
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11746-004-963-y